西北林学院学报2025，Vol.40Issue(3)：101-110,10.DOI:10.3969/j.issn.1001-7461.2025.03.12
基于UPLC-MS的柿叶红茶和柿叶绿茶差异性分析
Difference Analysis of Persimmon Leaf Black Tea and Green Tea Based on UPLC-MS
摘要
Abstract
To investigate the differences in antioxidant activities and metabolite characteristics between per-simmon leaf black tea and green tea,the total polyphenol,flavonoid contents,and antioxidant activities were determined by UV-visible spectrophotometry,and the metabolites were screened and identified in combination with ultra-high liquid chromatography-tandem mass spectrometry(UPLC-MS).The results showed that the total polyphenol,flavonoid contents,and the antioxidant activities of DPPH and ABTS radicals of persimmon leaf black tea were significantly higher than those of persimmon leaf green tea;a total of 199 differential metabolites were identified by UPLC-MS,with 7 categories,namely flavonoids,alkaloids,organic acids and their derivatives,nucleotides and their derivatives,and amino acids and their de-rivatives,lipids and phenolic acid,accounting for 22.61％,9.55％,8.04％,8.04％,7.04％,7.04％and 6.53％,respectively,of which the differences in the composition and content of flavonoids,alkaloids and phenolic acids may be an important reason for the differences of persimmon leaf black tea and persimmon leaf green tea.关键词
柿叶红茶/柿叶绿茶/抗氧化活性/差异代谢物/代谢组学Key words
persimmon leaf black tea/persimmon leaf green tea/antioxidant activity/differential metabo-lites/metabolomics分类
农业科技引用本文复制引用
胡银凤,王志勇,苗颐宁,韩卫娟,孙鹏,傅建敏..基于UPLC-MS的柿叶红茶和柿叶绿茶差异性分析[J].西北林学院学报,2025,40(3):101-110,10.基金项目
国家重点研发计划项目(2022YFD2200400 （）
2019YFD1001200). （）
