陕西科技大学学报2025，Vol.43Issue(3)：28-37,10.
工程纳米氧化锌暴露下的土壤微生物生态弹性评价
Study on the ecological resilience of soil microbiota under exposure to zinc oxide engineered nanoparticles
摘要
Abstract
Nano zinc oxide(ZnO),an essential nanomaterial,is extensively used in industrial and consumer products.This widespread application has significantly increased its environ-mental exposure and accumulation,necessitating a comprehensive evaluation of its ecological risks.This study evaluated the mid-term and long-term effects of ZnO engineered nanomate-rials(ZnO-ENs)on soil microorganisms and their ecological resilience through acute(200 mg/L,7 days)and chronic exposures at different levels(0,10,100,500 mg/kg for 30,90,and 120 days).Results indicated that under acute exposure,total protein content,total an-tioxidant capacity(T-AOC),and superoxide dismutase(SOD)activity of mixed cultures were significantly higher than those of pure cultures(p＜0.05),suggesting that microbial community diversity is fundamental to their ecological resilience.Carbon metabolic finger-print analysis revealed that the effects of ZnO-ENs were concentration-and time-dependent,with 10 mg/kg exposure showing no significant impact on microbial metabolic activity.Short-term exposure at 100 mg/kg significantly inhibited metabolic activity but exhibited some re-covery in long-term exposure,indicating partial ecological resilience.High concentration ex-posure(500 mg/kg)caused more significant and sustained effects.16S rDNA sequencing re-sults showed that short-term exposure to ZnO-ENs increased the abundance of Firmicutes,Actinobacteria,and Proteobacteria,while long-term exposure inhibited their growth and led to the emergence of new taxons including Ignavibacteriae,Elusimicrobia,and Chlorobi.Over-all,soil microbial communities exposed to low concentrations of ZnO-ENs demonstrated good ecological resilience under mid-term and long-term exposure.Medium concentration exposure showed partial functional recovery,whereas high concentration long-term exposure resulted in sustained significant effects on microbial metabolic functions and community structure,lac-king ecological resilience characteristics.关键词
氧化锌工程纳米颗粒/土壤微生物/碳代谢指纹图谱/生态弹性Key words
zinc oxide engineered nanoparticles/soil microbiota/carbon metabolic finger-printing/ecological resilience分类
环境科学引用本文复制引用
朱超,丁泽,陶坤,蒙芷芊,和苗,赵诣,南康康..工程纳米氧化锌暴露下的土壤微生物生态弹性评价[J].陕西科技大学学报,2025,43(3):28-37,10.基金项目
国家自然科学基金项目(22076113) （22076113）
