陕西科技大学学报2025，Vol.43Issue(3)：80-86,7.
产纤维素酶芽孢杆菌的筛选、鉴定及其发酵产酶条件优化
Screening,identification,and optimization of fermentation conditions for cellulase-producing Bacillus sp.
摘要
Abstract
Lignocellulose is the most abundant renewable resource in nature,and its biorefin-ery requires the use of cellulase for hydrolysis,but the low yield and high production costs significantly limit the large-scale industrial application of cellulase.To obtain high cellulase-producing bacterial strains,a cellulase-producing strain LT-4 was isolated and purified from the soil of sugarcane land in Liuzhou,Guangxi,by using Congo red-carboxymethyl cellulose sodium medium.The strain was then identified through molecular biology techniques,and the fermentation conditions for cellulase production were optimized using single-factor and or-thogonal experiments.The results showed that the strain LT-4 belongs to the genus Bacillus sp.Using Avicelase as the evaluation index,the optimal liquid fermentation conditions for cellulase production were as follows:Using a carbon source of wheat bran and sugarcane ba-gasse in a mass ratio of 4∶1,4 g/L of peptone as the nitrogen source,a fermentation broth pH of 8.0,and incubation at 39 ℃ for 36 h.After optimization,the optimized Avicelase activ-ity reached(0.237±0.014)U/mL,which is 2.3 times higher than that before optimiza-tion.This study is of great significance for improving cellulase yield and reducing production costs,providing a feasible solution for the efficient utilization and resource conversion of lignocellulose,while also laying a foundation for industrial applications in related fields.关键词
木质纤维素/纤维素酶/芽孢杆菌/液体发酵/优化Key words
lignocellulose/cellulase/Bacillus/liquid fermentation/optimization分类
轻工业引用本文复制引用
张婷,罗静雯,赖雪萍,冯东萍,黄春兰,韦志福,程忠..产纤维素酶芽孢杆菌的筛选、鉴定及其发酵产酶条件优化[J].陕西科技大学学报,2025,43(3):80-86,7.基金项目
国家自然科学基金项目(32460641) （32460641）
广西科技基地和人才专项项目(桂科AD24010031) （桂科AD24010031）
广西自然科学基金项目(2023GXNSFAA026235,2024GXNSFBA010330) （2023GXNSFAA026235,2024GXNSFBA010330）
广西南宁市"邕江计划"青年人才专项项目(RC20230202) （RC20230202）
国家级大学生创新创业训练计划项目(202411549029,202411549018) （202411549029,202411549018）
