现代中西医结合杂志2025，Vol.34Issue(8)：1050-1056,7.DOI:10.3969/j.issn.1008-8849.2025.08.005
骨化三醇对脑缺血再灌注损伤大鼠的保护作用及对线粒体自噬功能的影响
Calcitriol improves cerebral ischemia-reperfusion injury in rats by reducing mitochondrial autophagy
摘要
Abstract
Objective It is to explore the protective effect of calcitriol on cerebral ischemia-reperfusion injury in rats and its effect on mitochondrial autophagy.Methods Thirty-two SD rats were randomly divided into sham operation group,model group,normal saline group and osteotriol group,with 8 rats in each group.The osteotriol group was given osteotriol 2 μg/kg by gavage,and the model group and normal saline group were given normal saline 2 mL/kg by gavage,all once daily,continuously treated for 7 days.On the 8th day of the experiment,the rats of all groups except for the sham operation group were prepared to establish models of cerebral ischemia/reperfusion injury by wire suturing.The neurological deficits of the rats were evaluated by Longa's method,the cerebral infarction was observed by TTC staining,and the volume of cer-ebral infarction was calculated,the mitochondrial membrane potential was detected by JC-1 staining,the mitochondria ul-trastructure of cerebral cortex on the injured side was observed by transmission electron microscopy,and the expressions of mitochondrial function-related proteins NIP3-like protein X(NIX),microtubule-associated protein 1(Beclin1),dynamin-related protein 1(DRP1),optic atrophy 1(OPA1)and Peroxisome proliferator activated receptor gamma coactivator-1α(PGC-1α)in cerebral cortex on the injured side were measured by Western blotting.Results Compared with the sham op-eration group,the neurological deficit scores and cerebral infarction volumes of rats in the model group and normal saline group were significantly increased(all P<0.05);the red/green fluorescence intensity ratios in the mitochondrial mem-brane potential detection were significantly decreased(both P<0.05);the mitochondrial damage in the cerebral cortex was severe;the relative protein expressions of NIX,Beclin1 and DRP1 in cerebral cortex on the injured side were signifi-cantly increased(all P<0.05),while the relative protein expressions of OPA1 and PGC-1α were significantly reduced(all P<0.05).Compared with the model group and normal saline group,the neurological deficit scores and cerebral in-farction volume of rats in the osteotriol group were significantly reduced(all P<0.05);the red/green fluorescence intensi-ty ratios in the mitochondrial membrane potential decoction were significantly increased(both P<0.05);the mitochondrial damage in the cerebral cortex was obviously alleviated;the relative protein expressions of NIX,Beclin1 and DRP1 in cere-bral cortex on the injured side were significantly reduced(all P<0.05),and the relative protein expressions of OPA1 and PGC-1α were significantly increased(all P<0.05).Conclusion Osteotriol can protect brains of rats with cerebral ische-mia-reperfusion injury,which may maintain mitochondrial ultrastructure and thus protect neurons by modulating mitochon-drial autophagy,attenuating mitochondrial energy depletion,increasing mitochondrial biosynthesis and other processes.关键词
脑缺血再灌注损伤/大鼠/骨化三醇/线粒体自噬Key words
cerebral ischemia/reperfusion injury/rats/osteotriol/mitochondrial autophagy分类
医药卫生引用本文复制引用
杨静,邬军锋,徐逸轩,张云鹏,黄樱..骨化三醇对脑缺血再灌注损伤大鼠的保护作用及对线粒体自噬功能的影响[J].现代中西医结合杂志,2025,34(8):1050-1056,7.基金项目
上海市金山区卫健委课题(JSKJ-KTQN-2020-04) （JSKJ-KTQN-2020-04）
