西南林业大学学报2025，Vol.45Issue(3)：58-65,8.DOI:10.11929/j.swfu.202403078
萱草叶片大小相关基因挖掘与分析
Exploration and Analysis of Leaf Size Related Genes of Hemerocallis fulva
摘要
Abstract
To investigate the genes controlling leaf size of Hemerocallis fulva,the study conducted transcrip-tome sequencing and assembly on 2 varieties of H.fulva with different leaf sizes.A transcriptome library was constructed,followed by pathway analysis and functional annotation of differentially expressed genes.The find-ings revealed that a total of 90,366 gene clusters(unigenes),among which 10,671 unigenes showed significant dif-ferences in expression quantity.These unigenes accounted for 39.25％of the genetic sequences,with 6,483 genes upregulated(60.75％).By comparing these unigenes with 678 genes in NCBI database that are associated with leaf size and integrating references,the study identified 17 gene sequences that may control leaf size.Further confirm-ation through qRT-PCR revealed that ARF2,BRI1,CESA3,CYP90,IAA4 are closely associated with leaf develop-ment in H.fulva and play important roles in determining leaf size.关键词
萱草/叶片大小/转录组测序/基因Key words
Hemerocallis fulva/leaf size/transcriptional sequencing/gene分类
农业科技引用本文复制引用
陶桂祥,杨帆,严苓方,姚国琼,李伟,孙正海..萱草叶片大小相关基因挖掘与分析[J].西南林业大学学报,2025,45(3):58-65,8.基金项目
云南省教育厅科学研究基金项目(2019Y0152)资助 （2019Y0152）
云南省重点研发计划项目(202202AE090012-04)资助. （202202AE090012-04）
