西南林业大学学报2025,Vol.45Issue(3):66-75,10.DOI:10.11929/j.swfu.202405032
独蒜兰属叶绿体基因组序列及密码子偏好性分析
Analysis of Chloroplast Genome Sequence and Codon Preference of Pleione
摘要
Abstract
To clarify the usage pattern of codons in the chloroplast genome of Pleione,16 species of Pleione were used as study materials,and Codon W 1.4.2 and online software CUSP were used to obtain GC,RSCU,ENC and other relevant parameters of codons in Pleione,and neutral mapping,PR2-plot and optimal codon analysis were also carried out.The results showed that the contents of codons GC1,GC2,GC3 and GCall were similar in 16 species,and each species showed the following pattern:GC1>GC2>GC3,which indicated that the GC contents in the same species of Pleione were not uniformly distributed in different positions of the codons,and the codons preferred to end in A/U;the ENC values were all more than 35,which indicated that the codon preferences of Pleione were weak.It is evident that the formation of codon preferences in the chloroplast genomes of Pleione was mainly influenced by natural selection.The relative sequence usage(RSCU)and ENC values were used to identify the 10-22 optimal codons,most of which ended in A/U.关键词
独蒜兰属/叶绿体/基因组/密码子/偏好性Key words
Pleione/chloroplast/genome/codon/preference分类
农业科技引用本文复制引用
陶爱恩,尹成龙,李娅波,张玥,海永林,赵飞亚..独蒜兰属叶绿体基因组序列及密码子偏好性分析[J].西南林业大学学报,2025,45(3):66-75,10.基金项目
云南省科技厅基础研究计划面上项目(202101AT070001)资助 (202101AT070001)
云南省教育厅科学研究基金项目(2022J1214)资助 (2022J1214)
云南省大学生创新创业训练计划项目(SC20201332804,SC20211332803,SC202213328004)资助 (SC20201332804,SC20211332803,SC202213328004)
丽江市科技计划项目(2023LJSNK09)资助 (2023LJSNK09)
丽江市中青年学术和技术带头人后备人才项目资助 ()
校级中青年学术和技术带头人后备人才项目资助. ()