新医学2025，Vol.56Issue(5)：458-465,8.DOI:10.12464/j.issn.0253-9802.2025-0078
间充质干细胞与内皮细胞3D仿生分布设计促进组织工程支架血管化的效果
3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold
摘要
Abstract
Objective To fabricate 3D biomimetic design of tissue engineering scaffolds(Shell-Core scaffolds)incorporating mesenchymal stem cells(MSCs)and human umbilical vein endothelial cells(HUVECs)by using advanced coaxial 3D bioprinting technology,and to validate the biomimetic vascularization capacity of the scaffolds by experiments.Methods The Shell-Core scaffolds were successfully fabricated via coaxial 3D bioprinting technology.The biomimetic structural features were characterized using optical microscopy and histological staining assay.The in vitro pro-angiogenic capacity was evaluated through inter-group comparative observations and cell scratch assays.Furthermore,qRT-PCR was employed to quantify RNA expression levels of angiogenesis-related markers in cells cultured on the Shell-Core scaffolds.Results Shell-Core scaffolds were successfully fabricated.The scaffolds possessed high structural fidelity,which could be maintained at 7 d after scaffold fabrication.Cell proliferation assay showed that the cell proliferation rate in the scaffolds at 7 d was higher than that in the mixed culture on the 2D plane.Cell scratch assay showed that the shortening scratch distance of HUVECs treated by Shell-Core-CM was significantly greater than those treated by 3D-Mix-CM and blank groups[(431.6±33.6)μm vs.(378.7±22.5)μm vs.(302.3±20.1)μm,both P<0.01].At 7 d after in vitro cultured of engineered biomimetic tissues,under fluorescence microscope,HUVECs expressing green fluorescent protein remained in the designed core channel,and self-assembled endothelial buds in all directions.Furthermore,the results of qRT-PCR show that quantified RNA expression levels of angiogenesis-related markers(MMP-9)in cells cultured on the Shell-Core scaffolds was significantly higher(1.55±0.06,P<0.01)than that in 3D-Mix scaffolds.Conclusions The Shell-Core scaffolds integrating MSCs and HUVECs demonstrates high structural fidelity and pro-angiogenic capacity,offering a novel strategy for addressing tissue defect repair and fabricating vascularized engineered organs.This platform further provides a physiologically relevant tissue model for drug testing and mechanistic investigation of angiogenesis.关键词
同轴生物3D打印/预血管化/仿生设计/间充质干细胞/血管生成/组织修复Key words
Coaxial 3D bioprinting/Pre-vascularization/Biomimetic design/Mesenchymal stem cell/Angiogenesis/Tissue repair引用本文复制引用
谭佳梦,吴宇,陈建伟,朱德龙,王昆,朱蕾..间充质干细胞与内皮细胞3D仿生分布设计促进组织工程支架血管化的效果[J].新医学,2025,56(5):458-465,8.基金项目
广东省自然科学基金(2023A151501261) （2023A151501261）
