新医学2025，Vol.56Issue(5)：473-483,11.DOI:10.12464/j.issn.0253-9802.2025-0049
广州地区口腔鳞状细胞癌中EB病毒与人乳头状瘤病毒的感染及临床病理特征
Epstein-Barr virus and human papillomavirus infections and clinicopathological characteristics in oral squamous cell carcinoma in Guangzhou:a study based on specimens from affiliated hospitals of Sun Yat-sen University
摘要
Abstract
Objective To investigate the infection status and clinicopathological characteristics of Epstein-Barr virus(EBV)and human papillomavirus(HPV)in oral squamous cell carcinoma(OSCC)in Guangzhou,China.Methods A total of 304 OSCC specimens were collected from Guangzhou,including 123 cases from the Third Affiliated Hospital of Sun Yat-sen University(January 2014 to December 2024)and 181 cases from Sun Yat-sen Memorial Hospital of Sun Yat-sen University(January 2017 to December 2018).Polymerase Chain Reaction(PCR)was used to detect viral DNA,in situ hybridization was used to assess EBV-encoded RNAs(EBER),and immunohistochemistry was performed to identify viral-related proteins and determine the type of infection.Results Among the 304 OSCC specimens,26.3%(80/304)were EBV-PCR positive,with 63.8%(51/80)diagnosed at TNM stage I/II.Compared with EBV-negative cases,the difference in tumor staging was statistically significant(P=0.042).Among the EBV-PCR-positive OSCC cases,the EBER-positive rate was 33.8%(27/80),with 92.6%(25/27)localized in the cytoplasm.The detection rates of EBV-encoded nuclear antigen-1(EBNA-1),latent membrane protein-1(LMP-1),and EBV-encoded nuclear antigen-2(EBNA-2)were 83.8%(67/80),81.3%(65/80),and 56.3%(45/80),respectively,while the lytic protein ZEBRA was not detected.Type III latent infection accounted for 41.3%(33/80).In contrast,HPV DNA was detected in only 2.6%(8/304)of OSCC cases,among which two were p16-IHC positive.Only one case of OSCC showed EBV-HPV coinfection.Conclusions Based on samples collected from Sun Yat-sen University affiliated hospitals,EBV infection is relatively prevalent in OSCC cases in Guangzhou,mainly characterized by type III latency and cytoplasmic localization of EBER,suggesting a potential role of EBV in the pathogenesis of OSCC.In contrast,HPV-related OSCC appears to be rare and likely plays a limited pathogenic role.关键词
EB病毒/人乳头状瘤病毒/口腔鳞状细胞癌/潜伏感染/临床病理特征Key words
Epstein-Barr virus/Human papillomavirus/Oral squamous cell carcinoma/Latency infection/Clinicopathological characteristics引用本文复制引用
梁思泓,邵怡婷,李海刚,杜钰,纪燕英,班媛媛,胡雯,陈健宁,邵春奎..广州地区口腔鳞状细胞癌中EB病毒与人乳头状瘤病毒的感染及临床病理特征[J].新医学,2025,56(5):473-483,11.基金项目
广东省基础与应用基础研究基金(2024A1515012426,2022A1515012555,2021A1515110979) （2024A1515012426,2022A1515012555,2021A1515110979）
中国博士后科学基金(2023M744005) （2023M744005）
