云南民族大学学报（自然科学版）2025，Vol.34Issue(3)：253-260,8.DOI:10.3969/j.issn.1672-8513.2025.03.001
绿玉树内生真菌Cytospora sp.L2次生代谢产物及抑菌活性研究
Study on the secondary metabolites and antibacterial activity of endophytic fungus Cytospora sp.L2 from Euphorbia trucalli L.
摘要
Abstract
In order to study the secondary metabolites of the endophytic fungus from the Euphorbia trucalli L.,the endophytic fungus Cytospora sp.L2 from Euphorbia trucalli L.was isolated and subjected to solid fermentation,and the extracts from the solid fermentation were separated and purified by using modern separation methods such as silica gel and HPLC.A total of 10 compounds were obtained.The compounds were identified as six sterol compounds、two dimeric alkyls aromatic、one butenolide and one ketone derivative by nuclear magnetic spectrum analysis and other methods.The compounds were identified as dankasterones A(1),6β-hydroxystigmast-4-en-3-one(2),isocyathisterol(3),6β-hydroxy-ergosta-4,7,22-trien-3-one(4),(24R)-ergosta-4,6,8(14),22-tetraene-3-one(5),stigmast-4-en-3-one(6),integracin A(7),integracin B(8),methyl 2,5-dihydro-4-hydroxy-5-oxo-3-phenyl-2-furanpropanoate(9),4-methoxy-5-methoxycarbonyl-6-propyl-2-pyrone(10).Among them,compounds 1,3,5,6,9,and 10 were isolated from Cytospora sp.for the first time.In vitro antibacterial activity of the isolated compounds was determined by the double broth dilution method.The results showed that compounds 1-10 had weak inhibitory effects on Staphylococcus aureus,Staphylococcus epidermidis,Pseudomonas syringae pv.Tabaci and Ralstonia solanacearum.关键词
内生真菌/Cytospora sp./甾醇类化合物/抑菌活性/绿玉树Key words
endophytic fungus/Cytospora sp./steroid/bacteriostatic activity/Euphorbia trucalli L.分类
生物科学引用本文复制引用
陈宁,韦霁芮,杨富文,鲁松梅,邱仕瑜,陈世伟,周敏..绿玉树内生真菌Cytospora sp.L2次生代谢产物及抑菌活性研究[J].云南民族大学学报（自然科学版）,2025,34(3):253-260,8.基金项目
国家自科基金(31860099) （31860099）
云南省自然科学基金(202001AW07002). （202001AW07002）
