云南民族大学学报（自然科学版）2025，Vol.34Issue(3)：277-283,7.DOI:10.3969/j.issn.1672-8513.2025.03.004
HPLC法测定雀嘴茶中熊果苷的质量分数
Determination of the mass fraction of arbutin in Que Zui tea by HPLC
摘要
Abstract
A chromatographic column(250 mm×4.6 mm,5 μm)with octadecylsilane-bonded silica gel as the filler was used,with methanol and a volume fraction of 1%phosphoric acid aqueous solution as the mobile phase,to establish a method for determining the mass fraction of arbutin in tea using high-performance liquid chromatography(HPLC).The method demonstrates a good linear relationship within the mass concentration range of 0.349 to 402.788 μg/mL,with satisfactory precision,stability,and repeatability.Furthermore,the extraction method for arbutin in tea was optimized with respect to extraction procedures and solvents.This method not only provides a reliable analytical technique for determining the mass fraction of arbutin in tea but also offers new insights for detecting active ingredients in tea and other plant-based products.关键词
雀嘴茶/熊果苷/提取方法优化/质量分数测定Key words
Que Zui tea/arbutin/extraction method optimization/mass fraction determination分类
医药卫生引用本文复制引用
倪芹,王汝雄,李俊璇,来国防..HPLC法测定雀嘴茶中熊果苷的质量分数[J].云南民族大学学报（自然科学版）,2025,34(3):277-283,7.基金项目
云南省"生物医药领域"科技计划项目(2019ZF010). （2019ZF010）
