亚热带植物科学2025,Vol.54Issue(1):11-21,11.DOI:10.3969/j.issn.1009-7791.2025.01.002
不同基原与产地羌活挥发油成分差异性分析
Differential Analysis of Volatile Oil Composition of Notopterygii Rhizoma et Radix from Different Origins and Habitats
摘要
Abstract
The differences in volatile oil composition of Notopterygii Rhizoma et Radix(NRR)from different origins and habitats were studied to provide scientific basis for the quality control and resource development of NRR.Twenty batches of NRR herbs from different origins and habitats were used as experimental materials to extract the volatile oil by water vapour distillation.The volatile oil yields,compositions and relative contents of Notopterygium incisum(NI)and N.franchetii(NF)were systematically analyzed by GC-MS technology.The differences in chemical composition between NI and NF were interpreted from the perspective of volatile constituents by combining with cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).Results showed that the average volatile oil content of NI was higher than that of NF,whereas NF had a richer variety of volatile oil constituents.A total of 206 constituents were identified in the volatile oils from 20 batches,which were mainly hydrocarbons and alcohols.There were only 3 common constituents in 20 batches of the samples,which were all hydrocarbons.In addition,the 5 constituents with the highest contents,namely,β-pinene,(+)-α-pinene,(+)-limonene,p-mentha-1,4-diene,(-)-terpinen-4-ol were also significantly different in the different origins and habitats samples.The chemometrics analysis showed that HCA,PCA and OPLS-DA could effectively distinguish NI from NF,but could not be differentiated between samples of different habitats.A total of 23 differentiated volatile oil constituents were screened out using VIP>1 as a judgement criterion,which can be used as an important constituent for distinguishing NI from NF.There were significant differences in the types and contents of the volatile oils of NRR from different origins,but there were no obvious differences among different habitats,indicating that origins are the dominant factors among the many factors that cause differences in the volatile compositions of NRR.The results of this study provide a theoretical basis for the the quality evaluation system of NRR.关键词
羌活/宽叶羌活/基原/挥发油/GC-MS/差异Key words
Notopterygium incisum/N.franchetii/origins/volatile oil/GC-MS/difference分类
农业科学引用本文复制引用
张珺,李欠..不同基原与产地羌活挥发油成分差异性分析[J].亚热带植物科学,2025,54(1):11-21,11.基金项目
国家自然科学基金项目(32360113) (32360113)