亚热带植物科学2025，Vol.54Issue(1)：22-28,7.DOI:10.3969/j.issn.1009-7791.2025.01.003
多主棒孢霉对不同品种烟草的致病性及其培养条件优化研究
Pathogenicity of Corynespora cassiicola in Different Tobacco Cultivars and Optimization of Culture Conditions
摘要
Abstract
In order to elucidate the pathogenicity of Corynespora cassiicola and determine its optimization of cultivation conditions,this study conducted inoculation experiments using six common tobacco varieties inoculating with C.cassiicola strain HBU230811.The findings revealed varying levels of infection across all tested varieties;notably,Guizhou No.5 exhibited pronounced resistance against this strain.The ability for growth was observed across multiple culture media including PDA,MS,OA,KBA,LB,and TLA;although there were no discernible differences in colony diameter between KBA and OA,the size remained significantly larger compared to other media.On OA,mycelium appeared thinner whereas it displayed denser characteristics when grown on KBA,resulting in shorter spore-forming times.Moreover,the strain demonstrated normal growth within a pH range from 4 to 12,yet optimal conditions were observed at a pH level between 7 and 9.Additionally,various factors such as sugar sources,duration of light exposure,and temperature exerted significantly influences on the growth of C.cassiicola strain HBU230811.Notably,sorbitol as a sugar source combined with cultivation strain HBU230811.关键词
烟草/多主棒孢霉/培养条件优化/致病性/生长环境Key words
Nicotiana tabacum/Corynespora cassiicola/optimizing culture conditions/pathogenicity/growing environment分类
植物保护学引用本文复制引用
黄纯杨,杨相,周红江,史彩华,汪汉成,胡静荣..多主棒孢霉对不同品种烟草的致病性及其培养条件优化研究[J].亚热带植物科学,2025,54(1):22-28,7.基金项目
国家自然科学基金(32302343) （32302343）
贵州省烟草公司遵义市公司科技项目(2022XM09) （2022XM09）
