亚热带植物科学2025，Vol.54Issue(1)：37-44,8.DOI:10.3969/j.issn.1009-7791.2025.01.005
春石斛杂交种子非共生萌发研究
Non-symbiotic Germination of Hybrid Seeds of Dendrobium nobile
摘要
Abstract
In this paper,eight species of Dendrobium nobile were crossed and 13 hybrid combinations were formulated.Their F1 generation seeds were used as test material to investigate the effects of different capsule maturity and medium on the germination.And then the hormone concentrations of protocorm induction were optimized.The results showed that capsule maturity of 180-200 days was optimal for non-symbiotic germination of Dendrobium nobile hybrid seeds,as the short sprouting time and good sprouting at this stage.The germination rates of hybrid seeds in descending order on 5 different culture media are as follows:MS,N6,1/2MS,KC,B5.MS medium was the most suitable medium with a germination rate up to 90.27%.After seed germination,the differentiation of protocorms were good,and seedlings were formed quickly.The best medium for the differentiation of protocorm of Dendrobium nobile hybrid seeds was MS+NAA 0.2 mg·L-1+6-BA 1.0 mg·L-1,and the culture was transferred at 35-45 days.关键词
春石斛/杂交种子/非共生萌发Key words
Dendrobium nobile/hybrid seeds/non-symbiotic germination分类
生物学引用本文复制引用
刘靓,庄卫东,陈品品,卢建龙,武竞超..春石斛杂交种子非共生萌发研究[J].亚热带植物科学,2025,54(1):37-44,8.基金项目
泉州市科技计划项目(2020N011) （2020N011）
