针刺研究2025，Vol.50Issue(5)：567-575,9.DOI:10.13702/j.1000-0607.20240415
电针"天枢"改善小鼠结肠炎性反应的自主神经机制初探
Study on the underlying autonomic nervous mechanism of electroacupuncture in improving colonic inflammation in mice with chronic ulcerative colitis
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at"Tianshu"(ST25)on colonic inflammatory response in mice with ulcerative colitis(UC),so as to explore its underlying mechanisms in anti-inflammatory.Methods In the first part of this study,36 male C57BL/6J mice were randomly divided into normal,model,and EA groups(12 mice/group)according to their body weight.The UC mouse model was established by 3%dextran sulfate sodium(DSS)solution by free drinking for 7 consecutive days.EA(2 mA,10 Hz)was applied to ST25 for 30 min,once daily on the 1st,3rd,5th and 7th day.The body weight and colon length of mice were measured.HE staining was used to observe the pathological changes of colon tissue.The contents of interleukin(IL)-1,IL-6,tumor necrosis factor-α(TNF-α)and chemokine ligand 1(CXCL-1)in colon tissue were observed by MSD multiplex.In the second part of this study,9 mice were assigned to the normal,model and EA groups(3 mice/group).The UC model was established by free drinking of 3%DSS solution for 5 consecutive days.EA(2 mA,10 Hz)was applied to ST25 for 20 min on the 5th day.Immunofluorescence staining was used to detect the co-expression of ChAT+c-Fos in the dorsal vagal nucleus(DMV),T13—L1 segment of spinal cord and S2—S4 segment of sacral spinal cord.Results(1)Compared with the normal group,colon length and body weight were decreased(P<0.001)in mice of the model group,while the pro-inflammatory factors IL-1,IL-6,TNF-α and CXCL1 were significantly increased(P<0.01,P<0.001).Compared with the model group,colon length and body weight were increased(P<0.001)in mice of the EA group,while the contents of pro-inflammatory factors IL-6 and CXCL1 were decreased(P<0.01,P<0.05).H.E.staining showed severe damage of the colonic tissue with infiltration of a large number of inflammatory cells in the model group,which was milder in EA group.(2)Compared with the normal group,there was no significant difference in the expression levels of c-Fos in ChAT-positive neurons in the DMV region,and the lateral horns of T13—L1 and S2—S4 spinal cord in the model group.After the EA intervention,the expression levels of c-Fos in ChAT-positive neurons in the T13—L1 and S2—S4 lateral horns of the spinal cord were significantly increased compared with the model group(P<0.001,P<0.05).Conclusion EA at ST25 can effectively alleviate the inflammatory lesions and down-regulate the levels of pro-inflammatory cytokines in UC mice,which may be related to the activation of segmental sympathetic and/or sacral parasympathetic nervous innervating the colon,instead of the activation of descending vagal nerves.关键词
电针/天枢/溃疡性结肠炎/自主神经系统/神经-免疫调节Key words
Electroacupuncture/"Tianshu"(ST25)/Ulcerative colitis/Autonomic nervous system/Neuro-immune regulation引用本文复制引用
李桐,刘筱羽,万红叶,骆敏,张知云,王晓宇,曲正阳,宿杨帅,景向红..电针"天枢"改善小鼠结肠炎性反应的自主神经机制初探[J].针刺研究,2025,50(5):567-575,9.基金项目
国家自然科学基金项目(No.82274245、82105033) （No.82274245、82105033）
中国中医科学院优秀青年科技人才(创新类)培养专项(No.ZZ13-YQ-069) （创新类）
中国中医科学院针灸研究所创新人才专项(No.ZZ-JQ2023001) （No.ZZ-JQ2023001）
