针刺研究2025，Vol.50Issue(5)：576-583,8.DOI:10.13702/j.1000-0607.20240264
电针对帕金森病小鼠Wnt7a/β-catenin/GSK-3β通路介导的血脑屏障通透性的影响
Effect of electroacupuncture on the permeability of blood-brain-barrier mediated by Wnt7a/β-catenin/GSK-3β pathway in mice with Parkinson's disease
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on the permeability of blood-brain-barrier(BBB)and the regulation of the wingless-type mouse mammary tumor virus integration site 7a(Wnt7a)/β-catenin/glycogen synthase kinase-3β(GSK-3β)pathway in Parkinson's disease(PD)mice.Methods Thirty C57 BL/6 mice were randomly divided into normal,model and EA groups,with 10 mice in each group.The PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP,30 mg/kg),once daily for 7 days.In the EA group,acupuncture was applied to"Fengfu"(GV16),EA was applied to bilateral"Taichong"(LR3)and"Zusanli"(ST36)for 30 min,once daily for 7 days.The autonomic motor activity of mice in each group were observed by open-field test.The expression of tyrosine hydroxylase(TH)in substantia nigra was determined by immunohistochemistry.The permeability of BBB was detected by Evans blue staining.The expressions of Zona Occludens 1(ZO-1),Occludin,Claudin-5,Wnt7a,β-catenin and p-GSK-3β in substantia nigra were detected by Western blot.The positive cell expression of Wnt7a in substantia nigra was detected by immunofluorescence.Results Compared with the normal group,the behavioural score was increased(P<0.01),the total time of autonomic movement,the total distance and the average speed were decreased(P<0.01),the expression of TH in substantia nigra,the protein expressions of ZO-1,Occludin,Claudin-5,Wnt7a,β-catenin were down-regulated(P<0.01),the positive cell rate of Wnt7a was decreased(P<0.01)in the model group,while p-GSK-3β protein express and the content of Evans blue was increased(P<0.01).After EA intervention and compared with the model group,the behavioural score was decreased(P<0.01),the total time of autonomic movement,the total distance and the average speed were increased(P<0.01),the expressions of TH,ZO-1,Occludin,Claudin-5,Wnt7a and β-catenin were all up-regulated(P<0.01),the positive cell rate of Wnt7a was increased(P<0.01),while p-GSK-3β protein express and the content of Evans blue was decreased(P<0.01)in the EA group.Conclusion EA can effectively improve the motor dysfunction of PD mice,reduce the damage of dopaminergic neurons,and play a neuroprotective role.These effects may be associated with the up-regulation of tight junction proteins ZO-1,Occludin and Claudin-5,the regulation of key proteins in the Wnt7a/β-catenin/GSK-3β pathway,and the improvement of BBB permeability.关键词
帕金森病/电针/血脑屏障/紧密连接蛋白/无翅型小鼠乳腺肿瘤病毒整合位点7a/β-连环蛋白Key words
Parkinson's disease/Electroacupuncture/Blood-brain barrier/Tight junction protein/Wnt7a/β-catenin引用本文复制引用
张小蕾,胡梦妮,李亚楠,汪瑶,荣臻,祁羚,周仲瑜,马骏..电针对帕金森病小鼠Wnt7a/β-catenin/GSK-3β通路介导的血脑屏障通透性的影响[J].针刺研究,2025,50(5):576-583,8.基金项目
国家自然科学基金项目(No.81473788) （No.81473788）
