中国病理生理杂志2025，Vol.41Issue(5)：861-870,10.DOI:10.3969/j.issn.1000-4718.2025.05.004
Camp在小鼠肺癌进展中的作用及TGF-β/Smad通路介导EMT的分子机制研究
Role of Camp in progression of mouse lung cancer and molecular mecha-nism of EMT mediated by TGF-β/Smad pathway
摘要
Abstract
AIM:This study aims to investigate the role of cathelicidin-related antimicrobial peptide(Camp)gene in lung tumors in mice and to elucidate its molecular mechanisms in regulating epithelial-mesenchymal transition(EMT)and the TGF-β/Smad signaling pathway.METHODS:We conducted histological examinations and survival anal-yses using a KrasG12D spontaneous lung cancer mouse model with Camp gene knockout.This model was utilized to assess the impact of Camp on tumor nodule count,volume,and survival rate in mice.At the cellular level,we constructed A549-Camp and H1975-Camp cell lines to evaluate the effects of Camp on the proliferation,migration,and invasion of lung ade-nocarcinoma cells through soft agar colony formation assays,Scratch assays for cell migration rates,and Transwell assays.Additionally,we performed angiogenesis experiments to assess vascular development.At the molecular level,qRT-PCR was employed to detect the expression of angiogenic factors VEGF,bFGF,and TGF-β.Western blot analysis was utilized to examine the effects of Camp on the expression of EMT-related proteins(E-cadherin,N-cadherin,and Snail)and pro-teins associated with the TGF-β/Smad signaling pathway(TGF-β,p-TGF-β,Smad3,and Smad7).RESULTS:Camp gene knockout significantly suppressed lung tumorigenesis in mice,resulting in a decrease in tumor nodule count and vol-ume,as well as an enhancement in survival rates.In vitro,overexpression of Camp stimulated the proliferation,migra-tion,and invasion of lung adenocarcinoma cells.CONCLUSION:Molecular mechanism studies indicated that Camp modulated the expression of EMT-related proteins by influencing the TGF-β/Smad signaling pathway.关键词
cathelicidin相关抗菌肽/上皮-间充质转化/肺癌/血管生成Key words
cathelicidin-related antimicrobial peptide/epithelial-mesenchymal transition/lung cancer/an-giogenesis分类
临床医学引用本文复制引用
吴军录,李亚然,王林云,李锋..Camp在小鼠肺癌进展中的作用及TGF-β/Smad通路介导EMT的分子机制研究[J].中国病理生理杂志,2025,41(5):861-870,10.基金项目
国家自然科学基金青年项目(No.82304365) （No.82304365）
