中国病理生理杂志2025，Vol.41Issue(5)：902-908,7.DOI:10.3969/j.issn.1000-4718.2025.05.008
隐丹参酮通过JAK2/STAT3信号通路抑制异丙肾上腺素诱导的大鼠心肌肥厚
Cryptotanshinone attenuates isoproterenol-induced myocardial hypertro-phy in rats through JAK2/STAT3 signaling pathway
摘要
Abstract
AIM:To investigate the effect of cryptotanshinone(CPT)on myocardial hypertrophy induced by isoprenaline(ISO)in rats and explore its potential mechanism.METHODS:The experimental design consisted of two parts.The first aimed to investigate the effects of CPT on cardiac function,pathological manifestations,and the Janus ki-nase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway in rats with myocardial hyper-trophy.The rats were divided into six groups,namely the control,CPT control,and model groups and low-(15 mg·kg-1·d-1),medium-(30 mg·kg-1·d-1),and high-dose(60 mg·kg-1·d-1)CPT treatment groups,with six rats per group.The sec-ond part aimed to validate the role of the JAK2/STAT3 signaling pathway in the CPT-mediated myocardial hypertrophy treatment.Rats were divided into four groups,namely the control,model,high-dose CPT treatment,and coumermycin A1(CA1,a JAK2/STAT3 agonist)intervention(rats received ISO injection followed by 60 mg·kg-1·d-1 of high-dose CPT and 1 mg·kg-1·d-1 of CA1 for 15 d)groups,with five rats per group.Myocardial hypertrophy was induced in rats via intra-peritoneal injection of ISO(5 mg/kg),and CPT intervention lasted for 15 days.Cardiac function-related parameters were assessed using echocardiography,and pathological changes were evaluated through hematoxylin-eosin,Masson,and wheat germ agglutinin staining.Protein expression levels of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP),β-myosin heavy chain(β-MHC),and JAK2/STAT3 signaling pathway-related proteins were detected by Western blot analysis.RESULTS:Compared with the model group,CPT administration improved cardiac dysfunction-related ul-trasound markers and significantly reduced ISO-induced cardiomyocyte hypertrophy and myocardial fibrosis in rats with hy-pertrophy in a dose-dependent manner(P<0.05).Additionally,CPT decreased the levels of ANP,BNP,and β-MHC in-duced by ISO modeling(P<0.05),and inhibiting the phosphorylation of JAK2 and STAT3(P<0.05).Furthermore,a partial reversal of the therapeutic effect on myocardial hypertrophy induced by ISO modeling was observed when CA1 was administered(P<0.05).CONCLUSION:The CPT exhibits potential as a therapeutic agent for cardiac hypertrophy by effectively attenuating ISO-induced cardiac hypertrophy in rats through JAK2/STAT3 signaling inhibition.关键词
隐丹参酮/异丙肾上腺素/心肌肥厚/心肌重构/JAK2/STAT3信号通路Key words
cryptotanshinone/isoprenaline/myocardial hypertrophy/myocardial remodeling/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
刘丽娜,李春香,郭昌志,王群,赵焱,赵红晔,邓凤春..隐丹参酮通过JAK2/STAT3信号通路抑制异丙肾上腺素诱导的大鼠心肌肥厚[J].中国病理生理杂志,2025,41(5):902-908,7.基金项目
黑龙江省卫生健康委科研项目(No.20210303060359) （No.20210303060359）
齐齐哈尔市科技计划联合引导项目(No.LSFGG-2024075) （No.LSFGG-2024075）
齐齐哈尔医学科学院项目(No.QMSI2021B-13) （No.QMSI2021B-13）
