中国病理生理杂志2025，Vol.41Issue(5)：965-971,7.DOI:10.3969/j.issn.1000-4718.2025.05.015
基于TLR4/MyD88/NF-κB通路探索鳖甲煎丸治疗大鼠肝纤维化的作用机制
Mechanism of Biejiajian pill for treatment of liver fibrosis in rats based on TLR4/MyD88/NF-κB pathway
摘要
Abstract
AIM:To investigate the effect of Biejiajian pill(BJJP)in reducing carbon tetrachloride(CCl4)-in-duced liver fibrosis in rats and to explore the underlying mechanism of the TLR4-NF-κB signaling pathway.METHODS:A total of 40 male Wistar rats were randomly assigned to 4 groups:control group,model group,low-dose BJJP group,and high-dose BJJP groups,with 10 rats per group.The rat model of liver fibrosis was established by intraperitoneal injection of CCl4,and two doses of BJJP(2.2 g/kg and 0.55 g/kg)were administered simultaneously.After 8 weeks of modeling and BJJP administration,the enzyme-linked immunosorbent assay(ELISA)was used to measure liver injury indicators,including alanine aminotransferase(ALT)and aspartate aminotransferase(AST).Masson trichrome staining and Sirius red staining were performed to assess the fibrin deposition in liver tissue.Immunohistochemistry and Western blot analysis were conducted to detect the expression of extracellular matrix produced by hepatic stellate cells(HSCs).The expression of fibrosis-related proteins was examined using morphological assessment and Western blot.Finally,Western blot analysis was performed to detect the expression of proteins related to the Toll-like receptor 4(TLR4)/myeioid differentiation factor 88(MyD88)/NF-κB signaling pathway.RESULTS:The ELISA results indicated that BJJP treatment significantly re-duced ALT and AST levels in the serum of rats with liver fibrosis(P<0.01).Hematoxylin-eosin staining confirmed the protective effect of BJJP on liver tissue.Morphological analysis using Masson trichrome staining,Sirius Red staining,and alpha smooth muscle actin(α-SMA)immunohistochemical staining demonstrated that BJJP effectively reduced fibrin depo-sition in CCl4-induced rat liver fibrosis.Furthermore,the decreased expression of other markers associated with hepatic stellate cell activation,including fibronectin,collagen type Ⅰ,and α-SMA(P<0.01).Additionally,BJJP treatment sig-nificantly inhibited the activation of the TLR4/MyD88/NF-κB signaling pathway induced by CCl4(P<0.05).CONCLU-SION:BJJP alleviates CCl4-induced liver injury and fibrosis in rats.This effect may be attributed to its inhibition of the TLR4-NF-κB signaling pathway,which subsequently suppressed the HSCs activation.关键词
肝纤维化/鳖甲煎丸/TLR4/MyD88/NF-κB通路Key words
liver fibrosis/Biejiajian pill/TLR4/MyD88/NF-κB pathway分类
医药卫生引用本文复制引用
徐薇,王一钦,刘莉,庞杰..基于TLR4/MyD88/NF-κB通路探索鳖甲煎丸治疗大鼠肝纤维化的作用机制[J].中国病理生理杂志,2025,41(5):965-971,7.基金项目
国家自然科学基金资助项目(No.81704016) （No.81704016）
广东省中医药管理局项目(No.20231173 （）
No.20231662 （）
No.20231174) （）
