局解手术学杂志2025，Vol.34Issue(5)：379-385,7.DOI:10.11659/jjssx.04E024074
基于代谢组学研究高压电击后小鼠皮肤损伤的特点和机制
Study on the characteristics and mechanisms of skin damage in mice after high-voltage electric shock based on metabolomics
摘要
Abstract
Objective To study the damage effect of high-voltage electric shock on skin based on metabolomics,analyze its metabolic differences,and explore its injury mechanism.Methods A total of 16 SPF C57BL/6J male mice were divided into the electric shock group(head skin received electric shock treatment)and control group(head skin received electric shock acoustic-optical stimulation),and the skin appearance after treatment of mice in the two groups was observed.The histopathological changes caused by electric shock were analyzed by HE staining,EVG staining and Masson staining.GC-MS and LC-MS metabonomics were used to analyze the changes of skin metabolism spectrum and tissue metabolites after electric shock exposure,and the differential metabolites were analyzed.The obtained differential metabolites were combined and KEGG enrichment analysis was conducted.Results After high-voltage electric shock,the skin of mice could be damaged to the dermis,and the epidermis was partially thickened,lifted and separated.The structure of skin appendages in the dermis was destroyed,with a large number of inflammatory cells infiltrating and obvious swelling,accompanied by congestion,which led to severe skin inflammatory reaction and impaired skin barrier function.Metabonomics analysis suggested that the metabolites changed after electric shock exposure.KEGG enrichment analysis showed that electric shock significantly affected the central carbon metabolism pathway of cancer,pentose phosphate pathway,purine metabolism,glycine,serine and threonine metabolism processes,amino acid tRNA biosynthesis mechanism,glycerophospholipid metabolism pathway,pyrimidine metabolism pattern,glycolysis/gluconeogenesis,alanine metabolism process,glucagon signal pathway and so on.Conclusion High voltage electric shock can cause deep skin damage,disturb its energy metabolism and amino acid metabolism,and seriously interfere with its antioxidant and DNA repair system functions.关键词
电工作业/高压电击伤/皮肤损伤/代谢组学/KEGG富集分析Key words
electrician operation/high-voltage electric shock injury/skin damage/metabolomics/KEGG enrichment analysis分类
医药卫生引用本文复制引用
杨潇,邓平,陈思羽,李经典,王徽,岳洋,余争平,高鹏,皮会丰..基于代谢组学研究高压电击后小鼠皮肤损伤的特点和机制[J].局解手术学杂志,2025,34(5):379-385,7.基金项目
重庆英才包干制项目(cstc2024ycjh-bgzxm0102) （cstc2024ycjh-bgzxm0102）
重庆市科卫联合医学科研项目(重大项目)(2023DBXM006) （重大项目）
