中国临床药理学杂志2025，Vol.41Issue(6)：772-776,5.DOI:10.13699/j.cnki.1001-6821.2025.06.005
牛蒡子苷元对铜绿假单胞菌感染的支气管上皮细胞损伤的影响
Effects of Arctigenin ameliorates bronchial epithelial cell injury in Pseudomonas aeruginosa infection
摘要
Abstract
Objective To investigate the effect of arctigenin on bronchial epithelial cell injury caused by Pseudomonas aeruginosa(PA)infection and its mechanism.Methods BEAS-2B cells were randomly divided into control group(conventional culture),PA group(1.50×108 CFU·mL-1 PA infection),PA+low dose group(PA combined with 10.00 mmol·L-1 burdock aglycone),PA+high-dose group(PA combined with 20.00 mmol·L-1 burdock aglycone).The expression of inflammatory factors and glutathione(GSH)was detected by kit assay,glutathione peroxidase 4(GPX4)protein was detected by Western blot assay,and lipid peroxidation levels were detected by immunofluorescence assay.Results The IL-6 contents of control group,PA group,PA+low dose group and PA+high dose group were(20.43±2.82),(106.28±8.07),(76.66±5.73)and(50.57±3.98)pg·mL-1,respectively;the GSH contents were(25.95±1.46),(10.59±0.60),(13.98±0.74)and(21.52±2.36)ng·mL-1,respectively;GPX4 protein expression levels were 0.76±0.06,0.33±0.05,0.46±0.05 and 0.62±0.06,respectively;the lipid peroxidation levels were(100.00±4.29)％,(470.91±36.33)％,(331.16±27.04)％and(160.16±18.65)％,respectively.The above indexes in PA group compared with control group,PA+low dose group and PA+high dose group were compared with PA group,and the differences were all statistically significant(all P＜0.01).Conclusion Arctigenin can reduce the inflammation of PA-infected BEAS-2B cells by inhibiting GPX4-related ferroptosis pathway.关键词
牛蒡子苷元/铜绿假单胞菌/支气管上皮细胞/铁死亡/谷胱甘肽过氧化物酶4Key words
arctigenin/Pseudomonas aeruginosa/bronchial epithelial cells/ferroptosis/glutathione peroxidase 4分类
中医学引用本文复制引用
徐燕,王凯,沈萍..牛蒡子苷元对铜绿假单胞菌感染的支气管上皮细胞损伤的影响[J].中国临床药理学杂志,2025,41(6):772-776,5.基金项目
江苏省卫生健康委医学科研基金资助项目(X0566) （X0566）
