中国临床药理学杂志2025，Vol.41Issue(6)：777-783,7.DOI:10.13699/j.cnki.1001-6821.2025.06.006
川陈皮素对脂多糖诱导下牙周膜细胞的抗炎作用与细胞焦亡的研究
Research on the anti-inflammatory effects and pyroptosis of nobiletin on lipopolysaccharide-induced periodontal ligament cells
摘要
Abstract
Objective To explore the effect and mechanism of circular RNA RAS p21 protein activator 2(circRASA2)mediated by nobiletin(NOB)on lipopolysaccharide(LPS)-induced hPDLCs in periodontal ligament cells.Methods hPDLCs cells were divided into control group,model group(hPDLCs cells were treated with 10 μg·mL-1 LPS for 24 h),NOB-H group(Based on the model group,20 μmol·L-1 NOB was given for 24 h),NOB+oe-NC group(hPDLCs cells transfected with oe-NC were treated with 20 μmol·L-1 NOB)and NOB+oe-circRASA2 group(hPDLCs cells transfected with oe-circRASA2 were treated with 20 μmol·L-1 NOB).Quantitative real time polymerase chain reaction was used to detect the mRNA expression levels of circRASA2.The secretion levels of inflammation-related factors were detected by enzyme-linked immunosorbent assay.Western blot analysis of slit homolog 2(Slit2)/mitogen-activated protein kinase(MAPK)signaling pathway related protein expression levels.Results The relative expression levels of circRASA2 in control group,model group and NOB-H group were 1.00±0.15,2.83±0.50 and 1.36±0.27,respectively.The levels of interleukin-1β in control group,model group,NOB-H group,NOB+oe-NC group and NOB+oe-circRASA2 group were(144.82±24.89),(367.54±65.73),(228.51±40.24),(219.78±36.94)and(324.25±61.57)mg·mL-1,respectively;interleukin-18 levels were(167.50±27.33),(394.68±74.96),(212.47±39.51),(205.93±41.18)and(358.11±66.27)mg·mL-1,respectively;the relative expression levels of Slit2 protein were 1.00±0.18,2.35±0.41,1.61±0.29,1.57±0.27 and 2.14±0.38,respectively;the ratios of phospho-p38 mitogen-activated protein kinase/p38 mitogen-activated protein kinase protein were 1.00±0.16,2.08±0.37,1.39±0.25,1.33±0.21 and 1.92±0.34,respectively.The above indexes in the model group were compared with the control group,the NOB-H group was compared with the model group,and the NOB+oe-circRASA2 group was compared with the NOB+oe-NC group,and the differences were statistically significant(all P＜0.05).Conclusion NOB alleviates LPS-induced inflammation and inhibits pyroptosis in hPDLCs cells by inhibiting circRASA2 expression,possibly by inhibiting Slit2/MAPK signaling pathway.关键词
川陈皮素/环状RNA RAS p21蛋白激活因子2/牙周膜细胞/细胞焦亡/SLIT同源物2/丝裂原活化蛋白激酶信号通路Key words
nobiletin/circular RNA RAS p21 protein activator 2/periodontal ligament cell/pyroptosis/slit homolog 2/mitogen-activated protein kinase pathway分类
中医学引用本文复制引用
李淑华,刘安东..川陈皮素对脂多糖诱导下牙周膜细胞的抗炎作用与细胞焦亡的研究[J].中国临床药理学杂志,2025,41(6):777-783,7.基金项目
安徽省高校自然科学研究一般项目基金资助项目(ZR2022B008) （ZR2022B008）
