摘要
Abstract
Objective To investigate the effect of anemoside B4 on the malignant biological behavior of non-small cell lung cancer(NSCLC)cells mediated by microRNA-142-3p(miR-142-3p)on the expression of high mobility group protein A1(HMGA1).Methods A549 cells were divided into A549 group(normal culture),anemoside B4 group(100 μg·mL-1 anemoside B4 treatment),inhibitor NC group(100 μg·mL-1 anemoside B4+transfection inhibitor NC treatment),miR-142-3p inhibitor group(100 μg·mL-1 anemoside B4+transfected with miR-142-3p inhibitor treatment),si-NC group(100 μg·mL-1 anemoside B4+co-transfected with miR-142-3p inhibitor and si-NC treatment),si-HMGA1 group(100 μg·mL-1 anemoside B4+co-transfected with miR-142-3p inhibitor and si-HMGA1 treatment).Real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blotting(WB)were used to detect the expression of miR-142-3p and HMGA1.Cell proliferation,migration and apoptosis were detected by 5-acetylidene-2'-deoxyuracil riboside(Edu),Transwell and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay(TUNEL).Results The miR-142-3p relative expression levels of A549 group,anemoside B4 group,inhibitor NC group and miR-142-3p inhibitor group were 1.00±0.14,1.58±0.18,1.60±0.21 and 1.12±0.16,respectively;the relative expression levels of HMGA1 protein were 1.00±0.23,0.56±0.07,0.59±0.08 and 0.95±0.21,respectively;the proliferation rates were(84.62±9.11)％,(62.15±7.20)％,(63.89±7.77)％and(76.35±8.11)％,respectively;the migration number was(156.23±17.54),(80.65±8.67),(82.15±8.96)and(111.55±12.75)cells,respectively;the apoptosis rates were(8.96±0.95)％,(32.56±3.56)％,(33.95±3.79)％and(16.89±1.92)％,respectively.The proliferation rates of si-NC group and si-HMGA1 group were(75.98±8.05)％and(64.52±7.25)％,respectively;the migration number was(118.85±12.98)and(86.95±9.21)cells,and the apoptosis rates were(17.58±2.02)％and(28.15±3.13)％,respectively.The above indexes in A549 group were compared with anemoside B4 group,miR-142-3p inhibitor group were compared with inhibitor NC group,si-HMGA1 group were compared with si-NC group,the differences were all with statistically significant(all P＜0.05).Conclusion Anemoside B4 may inhibit proliferation and migration of NSCLC cells and promote apoptosis by regulating the miR-142-3p/HMGA1 axis.关键词
白头翁皂苷B4/高迁移率组A1/非小细胞肺癌/细胞凋亡/生物学行为Key words
anemoside B4/high mobility group protein A1/non-small cell lung cancer/cell apoptosis/biological behavior分类
医药卫生
