中国临床药理学杂志2025，Vol.41Issue(6)：790-793,4.DOI:10.13699/j.cnki.1001-6821.2025.06.008
靶向GAGE12I的miR-28-5p体外抑制胃癌细胞增殖、迁移和侵袭的研究
MiR-28-5p targeting GAGE12I inhibits gastric cancer cell proliferation,migration and invasion in vitro
摘要
Abstract
Objective To investigate the in vitro inhibitory effects of microRNA-28-5p(miR-28-5p)targeting G antigen 12I antibody(GAGE 12I)on the proliferation,migration,and invasion of gastric cancer cells.Methods SGC-7901 cell were randomly divided into miR-28-5p group(miR-28-5p mimic),miR-NC group(miR-28-5p negative control mimic),shGAGE12I group(GAGE12I knockout)and blank group(GAGE12I not knockout).Cell proliferation,migration,and invasion capabilities were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)colorimetric assay and invasion chambers.Bioinformatics analysis and luciferase activity assays were conducted to examine the interaction between miR-28-5p and GAGE12I.Results The proliferation rates of miR-28-5p and miR-NC groups were(99.90±10.30)％and(48.25±4.15)％;the invasion rates were(145.57±14.52)％and(67.30±6.95)％;the mobility rates were(103.58±10.75)％and(43.18±4.26)％,respectively,the differences were statistically significant(all P＜0.05).The proliferation rates of shGAGE12I group and blank group were(99.80±10.20)％and(35.50±3.58)％;the invasion rates were(142.70±13.65)％and(65.30±6.75)％;the mobility rates were(103.55±10.06)％and(48.38±4.52)％,respectively,the differences were statistically significant(all P＜0.05).GAGE12I was the target of miR-28-5p.Conclusion MiR-28-5p negatively regulates GAGE12I and reduces the proliferation,invasion and migration of gastric cancer cells.关键词
G抗原12I抗体/微小核糖核酸-28-5p/胃癌/增殖/迁移/侵袭研究Key words
G antigen 12I antibody/microRNAs-28-5p/gastric cancer/proliferation/migration/invasion studies分类
医药卫生引用本文复制引用
肖丽萍,曾庆芳,詹涛..靶向GAGE12I的miR-28-5p体外抑制胃癌细胞增殖、迁移和侵袭的研究[J].中国临床药理学杂志,2025,41(6):790-793,4.基金项目
江西省卫健委科技计划基金资助项目(202311986) （202311986）
