Abstract
Objective To explore the effects of aloe-emodin on the proliferation,apoptosis,and epithelial-mesenchymal transition(EMT)of pathological scar fibroblasts,as well as its regulatory mechanisms.Methods Proliferative scar fibroblasts(HSFb)were randomly divided into the following groups:HSFb control group(normal culture),HSFb low-dose group(25.00 μg·mL-1 aloe-emodin),HSFb medium-dose group(50.00 μg·mL-1 aloe-emodin),HSFb high-dose group(100.00 μg·mL-1 aloe-emodin),and HSFb-PFT-α group(100.00 μg·mL-1 aloe-emodin+20.00 μmol·L-1 p53-specific inhibitor PFT-α).Keloid fibroblast(KFb)cells were randomly divided into the following groups:KFb control group(normal culture),KFb low-dose group(25.00 μg·mL-1 aloe-emodin),KFb medium-dose group(50.00 μg·mL-1 aloe-emodin),KFb high-dose group(100.00 μg·mL-1 aloe-emodin),and KFb-PFT-α group(100.00 μg·mL-1 aloe-emodin+20.00 μmol·L-1 p53-specific inhibitor PFT-α).Cell viability was assessed using the cell counting kit-8(CCK-8)assay,Western blotting was performed to detect the expression of p53 and EMT-related proteins,apoptosis was measured using the TUNEL assay.Results The cell survival rates of the KFb control group,KFb low-dose group,KFb medium-dose group,and KFb high-dose group were(100.00±5.17)％,(84.13±8.34)％,(73.81±7.62)％and(54.59±3.47)％,respectively;the cell survival rates of the HSFb control group,HSFb low-dose group,HSFb medium-dose group,and HSFb high-dose group were(100.00±6.77)％,(83.50±6.08)％,(71.43±2.70)％and(61.80±4.79)％,respectively.The relative expression levels of p53 protein in the KFb control group,KFb low-dose group,KFb high-dose group,and KFb-PFT-α group were 0.31±0.04,0.52±0.04,0.76±0.06 and 0.38±0.05,respectively;the relative expression levels of E-cadherin protein were 0.35±0.04,0.58±0.08,0.76±0.13 and 0.41±0.06,respectively;the apoptosis rates were(4.82±0.59)％,(11.72±1.68)％,(20.36±1.93)％and(12.46±1.28)％,respectively;the relative expression levels of p53 protein in the HSFb control group,HSFb low-dose group,HSFb high-dose group,and HSFb-PFT-α group were 0.37±0.03,0.48±0.05,0.65±0.07 and 0.46±0.04,respectively;the relative expression levels of E-cadherin protein were 0.26±0.03,0.46±0.05,0.63±0.08 and 0.34±0.05,respectively;the apoptosis rates were(4.89±0.28)％,(13.98±0.83)％,(21.51±1.08)％and(12.95±1.10)％,respectively.The differences between the KFb low-and high-dose groups and the KFb control group,the KFb-PFT-α group and the KFb high-dose group,the HSFb low-and high-dose groups and the HSFb control group,and the HSFb-PFT-α group and the HSFb high-dose group were statistically significant(P＜0.05,P＜0.01,P＜0.001).Conclusion Aloe-emodin may inhibit the proliferation of pathological fibroblasts,EMT,and induce apoptosis by regulating the p53-related signaling pathway.关键词
芦荟大黄素/病理性瘢痕/成纤维细胞/上皮间质转化/p53信号通路Key words
aloe-emodin/pathological scar/fibroblast/epithelial mesenchymal transformation/p53 signal pathway分类
医药卫生
