中国临床药理学杂志2025，Vol.41Issue(6)：800-804,5.DOI:10.13699/j.cnki.1001-6821.2025.06.010
槲皮素在急性骨骼肌损伤中促进肌肉再生的研究
Research of quercetin promote muscle regeneration in acute skeletal muscle injury
摘要
Abstract
Objective To investigate the mechanism of quercetin in promoting muscle regeneration in acute skeletal muscle injury.Methods Satellite cells were randomly divided into blank group(conventional culture),hydrogen peroxide(H2O2)group(100 μmol·L-1 H2O2 treated cells for 24 h),quercetin group(10 μmol·L-1 quercetin treated cells for 24 h),H2O2+quercetin group(100 μmol·L-1 H2O2+10 μmol·L-1 quercetin treated cells for 24 h).Cell proliferation was detected by cell counting kit-8(CCK-8)method;protein expression was detected by Western blot assay;mitochondrial reactive oxygen species(ROS)levels and related indicators of mitochondrial respiration were detected by kit.Results The 24 h proliferation rates of cells in blank group,quercetin group,H2O2 group and H2O2+quercetin group were(100.00±4.27)％,(148.79±13.17)％,(81.70±3.19)％and(120.83±8.81)％,respectively;the relative expression levels of superoxide dismutase 1(SOD1)protein were 0.64±0.09,0.76±0.09,0.24±0.03 and 0.43±0.06,respectively;the relative expression levels of myosin heavy chain(MyHC)protein were 0.61±0.05,0.67±0.06,0.32±0.03 and 0.47±0.06,respectively;the fluorescence intensities of mitochondrial ROS were(52.56±1.97),(48.46±4.05),(119.53±13.06)and(102.91±8.93)a.u.,respectively;the catalase(CAT)levels were(28.66±2.18),(35.62±3.89),(18.13±1.78)and(23.97±2.72)μmol·mg protein-1,respectively;mitochondrial respiratory chain(MRC)complex Ⅰ levels were(19.77±1.01),(23.28±2.48),(9.87±1.01)and(14.19±0.75)μmol·mg protein-1,respectively.The above indexes in quercetin group and H2O2 group were compared with blank group,the above indexes in H2O2+quercetin group were compared with H2O2 group,the difference was statistically significant(P＜0.05,P＜0.01,P＜0.001).Conclusion Quercetin can promote mitochondrial respiration by clearing mitochondrial ROS levels,improve oxidative damage,and then promote satellite cell proliferation,which can promote muscle regeneration in acute skeletal muscle injury.关键词
槲皮素/急性骨骼肌损伤/肌肉再生/卫星细胞/氧化损伤/线粒体Key words
quercetin/acute skeletal muscle injury/muscle regeneration/satellite cells/oxidative damage/mitochondria分类
医药卫生引用本文复制引用
袁玉鹏,江亚..槲皮素在急性骨骼肌损伤中促进肌肉再生的研究[J].中国临床药理学杂志,2025,41(6):800-804,5.基金项目
安徽省高校优秀人才培育基金资助项目(gxgwfx2020012) （gxgwfx2020012）
安徽省高校哲学社会科学研究基金资助项目(2023AH051265) （2023AH051265）
