中国临床药理学杂志2025，Vol.41Issue(6)：805-810,6.DOI:10.13699/j.cnki.1001-6821.2025.06.011
灵孢多糖通过调控Nrf2/HO-1信号途径减轻H2O2诱导小鼠海马神经元细胞铁死亡的研究
Research on GLPS alleviated H2O2-induced ferroptosis in mouse hippocampal neurons by regulating Nrf2/HO-1 signaling pathway
摘要
Abstract
Objective To investigate the effect of ganoderma lucidum polysaccharides(GLPS)on ferroptosis of hippocampal neuronal(HT22)cell lines induced by hydrogen peroxide(H2O2)and its mechanism.Methods The H2O2-induced HT22 cell injury model was first established in vitro.HT22 cells were divided into normal group(conventional culture),H2O2 group(200 μmol·L-1 H2O2),GLPS group(200 μmol·L-1 H2O2+100 μg·mL-1 GLPS),H2O2+Fin56 group(200 μmol·L-1 H2O2+5 μmol·L-1 Fin56),H2O2+Fin56+GLPS group(200 μmol·L-1 H2O2+5 μmol·L-1 Fin56+100 μg·mL-1 GLPS).The oxidative stress markers were detected by enzyme-linked immunosorbent assay(ELISA);the protein expression levels of iron death markers,nuclear factor erythroid2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)were detected by Western blot.Results The contents of malondialdehyde(MDA)in normal group,H2O2 group,GLPS group,H2O2+Fin56 group and H2O2+Fin56+GLPS group were(5.56±0.61),(15.53±0.83),(6.51±0.29),(20.92±1.03)and(13.65±0.70)nmol·mL-1;the contents of superoxide dismutase(SOD)were(37.93±1.37),(22.48±0.59),(37.81±2.56),(16.10±2.28)and(22.21±2.99)μg·mL-1;Ferritin protein expression levels were 1.52±0.03,1.22±0.07,1.42±0.06,0.64±0.02 and 0.93±0.13;GPX4 protein expression levels were 3.29±0.34,1.91±0.18,2.99±0.22,1.13±0.07 and 2.45±0.05;Nrf2 protein expression levels were 0.90±0.01,2.24±0.02,2.97±0.07,2.45±0.07 and 2.76±0.11;HO-1 protein expression levels were 1.58±0.02,1.76±0.04,2.29±0.06,1.67±0.02 and 2.03±0.06,respectively.H2O2 group was compared with normal group and H2O2+Fin56 group,GLPS group was compared with H2O2 group,H2O2+Fin56+GLPS group was compared with H2O2+Fin56 group and GLPS group,the differences were statistically significant(P＜0.05,P＜0.01).Conclusion GLPS can improve lipid peroxide accumulation and iron metabolism disorder induced by H2O2 in HT22 cells by activating Nrf2/HO-1 pathway,thereby inhibiting the occurrence of ferroptosis.关键词
灵孢多糖/小鼠海马神经元细胞/过氧化氢/铁死亡/脂质过氧化/核因子E2相关因子2/血红素加氧酶-1通路Key words
ganoderma lucidum polysaccharides/hippocampal neuronal cell line/hydrogen peroxide/ferroptosis/lipid peroxidation/nuclear factor erythroid2-related factor 2/heme oxygenase-1 pathway分类
医药卫生引用本文复制引用
李彦青,杨智超,宋丽娟,王青,王翰斌,杨立志,肖保国,马存根..灵孢多糖通过调控Nrf2/HO-1信号途径减轻H2O2诱导小鼠海马神经元细胞铁死亡的研究[J].中国临床药理学杂志,2025,41(6):805-810,6.基金项目
国家自然科学基金资助项目(82304752) （82304752）
山西省横向课题资助项目(2022HX-GR-01) （2022HX-GR-01）
山西省基础研究计划(自由探索类)青年基金资助项目(20210302123312) （自由探索类）
山西省卫生健康科研课题资助项目(2022127) （2022127）
山西省卫健委医学科技领军团队基金资助项目(2020TD05) （2020TD05）
优秀博士毕业生来晋工作奖励经费科研启动基金资助项目(2021BKS01) （2021BKS01）
山西中医药大学博士科研启动基金资助项目(2022BK15) （2022BK15）
山西中医药大学科技创新能力培育计划"基础研究专项"基金资助项目(2021PY-JC-15) （2021PY-JC-15）
山西中医药大学学科建设经费资助项目(030200117) （030200117）
