中国临床药理学杂志2025，Vol.41Issue(6)：811-815,5.DOI:10.13699/j.cnki.1001-6821.2025.06.012
七叶皂苷钠对人脐带间充质干细胞向神经样细胞分化的诱导作用
Effects of sodium aescinate on the differentiation of human umbilical cord mesenchymal stem cells into neural-like cells
摘要
Abstract
Objective To investigate the effects of sodium aescinate on the differentiation of human umbilical cord mesenchymal stem cells(HUMSC)into neural-like cells and its possible mechanism.Methods HUMSC cells were randomly divided into blank group,experiment-L group,experiment-M group and experiment-H group.All the cells were cultured with neurobasal medium(NM)medium,cells in experiment-L group,experiment-M group and experiment-H group were treated with 1.00,2.00 and 4.00 mg·L-1 sodium aescinate,respectively.The expression levels of neuron-like cells markers and synaptic markers were detected by reverse transcription-polymerase quantitative chain reaction(RT-qPCR)and Western blot.Results The relative mRNA expression levels of microtubule-associated protein 2(MAP-2)in blank group,experiment-L group,experiment-M group and experiment-H group were 1.00±0.14,1.26±0.08,1.74±0.16 and 2.30±0.20,respectively;the relative mRNA expression levels of growth associated protein 43(GAP43)were 1.00±0.10,1.14±0.15,1.34±0.15 and 1.53±0.11,respectively;the relative expression levels of synaptophysin(SYN)mRNA were 1.00±0.09,1.15±0.08,1.32±0.12 and 1.48±0.13,respectively;the expression levels of neurofilament heavy chain(NF-H)protein were 0.41±0.04,0.50±0.06,0.71±0.06 and 1.21±0.09,respectively;postsynaptic density protein 95(PSD95)protein expression levels were 0.27±0.04,0.41±0.06,0.52±0.04 and 0.68±0.06,respectively;silent information regulator 1(SIRT1)protein expression levels were 0.31±0.04,0.52±0.06,0.67±0.06 and 0.80±0.10,respectively.The above indexes in experiment-L group,experiment-M group and experiment-H group were compared with blank group,the differences were all statistical significant(P＜0.05,P＜0.01);the above indexes compared among experiment-L group,experiment-M group and experiment-H group,the differences were all statistically significant(P＜0.05,P＜0.01).Conclusion Sodium aescinate may induce the differentiation of HUMSC into neural-like cells by regulating SIRT1 in vitro.关键词
七叶皂苷钠/脐带间充质干细胞/神经样细胞/分化/沉默信息调节因子1Key words
sodium aescinate/umbilical cord mesenchymal stem cell/nerve-like cell/differentiation/silent information regulator 1分类
医药卫生引用本文复制引用
潘晓冉,朱勇,周坚,向光红..七叶皂苷钠对人脐带间充质干细胞向神经样细胞分化的诱导作用[J].中国临床药理学杂志,2025,41(6):811-815,5.基金项目
湖南中医药大学校级科研基金资助项目(2022XYLH087) （2022XYLH087）
