中国水稻科学2025，Vol.39Issue(3)：322-330,9.DOI:10.16819/j.1001-7216.2025.240304
水稻类病斑突变体lm52的基因克隆及其广谱抗病性分析
Gene Cloning and Broad-spectrum Disease Resistance Analysis of Rice Lesion Mimic Mutant lm52
摘要
Abstract
[Objective]Lesion mimic mutants are ideal materials to study the mechanisms of plant cell death and disease resistance,offering new strategies for plant disease resistance breeding.[Method]The lm52 mutant gene was cloned by the map-based cloning method,and the function of the candidate gene was verified by CRISPR/Cas9 knockout technology.Meanwhile,the resistance of lm52 to multiple rice pathogens and the possible resistance mechanisms were analyzed.[Result]The mutant lm52 gradually developed progressive brown spot lesions from the 4th leaf stage,with whole leaf necrosis at maturity stage.A single base mutation(G1440A)on the ATPase encoding gene LOC_Os06g03940 caused the lesion mimic phenotype of lm52.lm52 is characterized by significantly enhanced resistance to blast,sheath blight and bacterial blight,which was found to be related to the overaccumulation of ROS and the activation of defense-related genes.[Conclusion]The mutation of the ATPase gene LM52 enhances rice resistance against diverse pathogens,and provides potential target genes for molecular breeding of rice disease resistance.关键词
水稻/类病斑突变体/图位克隆/抗病性Key words
rice/lesion mimic mutant/map-based cloning/disease-resistance引用本文复制引用
黄涛,冯志明,左示敏,魏兆根,陈玘,程泽,刘欣,王广达,胡珂鸣,谢文亚,陈宗祥..水稻类病斑突变体lm52的基因克隆及其广谱抗病性分析[J].中国水稻科学,2025,39(3):322-330,9.基金项目
江苏省农业科技自主创新资金项目[CX(23)3001] （23）
江苏省政府种业振兴计划资助项目[JBGS(2021)040] （2021）
国家自然科学基金资助项目(32272110) （32272110）
扬州大学大学生创新创业训练计划项目(XCX20230650) （XCX20230650）
扬州大学"青蓝工程"资助项目. （）
