中国水稻科学2025，Vol.39Issue(3)：331-342,12.DOI:10.16819/j.1001-7216.2025.2401010
水稻真核翻译起始因子OseIF6.2调控粒型的功能研究
Functional Study of Rice Eukaryotic Translation Initiation Factor OseIF6.2 in Grain Size Regulation
摘要
Abstract
[Objective]This study aims to investigate the functional role of the rice eukaryotic translation initiation factor OseIF6.2 in rice growth and development using a reverse genetics approach,thereby laying a foundation for further exploration of the functions of OseIF6.2.[Method]First,bioinformatics methods were employed to perform multiple sequence alignment and a phylogenetic tree was constructed to analyze the evolutionary relationship between OseIF6.2 and eIF6 proteins in other eukaryotes.CRISPR/Cas9 gene editing technology was used to precisely edit the OseIF6.2 gene in rice(Nipponbare).Next,the expression levels of OseIF6.2 in different tissues of rice were detected using quantitative real-time PCR(qRT-PCR)technology.The precise localization of OseIF6.2 within cells was observed using a confocal laser microscope.Finally,the interaction between OseIF6.2 and its potential interacting protein,OseIF6.1,was validated using yeast two-hybrid(Y2H)and bimolecular fluorescence complementation(BiFC)assays.[Result]Phylogenetic analysis revealed that OseIF6.2 is highly conserved among eukaryotic eIF6 proteins,underscoring its universal role in translation regulation.qRT-PCR results indicated that OseIF6.2 expression was relatively high in rice stems.Subcellular localization analysis using GFP-tagged OseIF6.2 in tobacco leaf cells showed fluorescence signals in both the nucleus and cytoplasm,confirming its dual localization.Additionally,compared to the wild type,the oseif6.2 mutant exhibited significant reductions in grain length,grain thickness,and 1000-grain weight.Both Y2H and BiFC experiments confirmed a direct interaction between OseIF6.2 and OseIF6.1[Conclusion]In summary,OseIF6.2,a eukaryotic translation initiation factor in rice,plays a crucial role in regulating grain morphology,further highlighting its importance in rice growth,development,and grain formation.关键词
真核翻译起始因子/OseIF6.2/水稻/粒型/生长发育Key words
eukaryotic translation initiation factor/OseIF6.2/rice/grain shape/growth and development引用本文复制引用
马顺婷,任光俊,郭鸿鸣,胡运高,高方远,刘利平,牟昌铃,吕建群,苏相文,刘松,梁毓玉..水稻真核翻译起始因子OseIF6.2调控粒型的功能研究[J].中国水稻科学,2025,39(3):331-342,12.基金项目
四川省农业科学院农业前沿技术攻关专项(5+1QYGG003) （5+1QYGG003）
国家现代农业产业技术体系四川创新团队项目(SCCXTD-2024-1) （SCCXTD-2024-1）
国家重点研发计划资助项目(2024YFD1200900) （2024YFD1200900）
国家现代农业产业技术体系建设专项(CARS-01) （CARS-01）
四川省农业科学院原始创新项目(YSCX2035-010) （YSCX2035-010）
四川省科技计划重点研发项目(2021YFYZ0016). （2021YFYZ0016）
