中国水稻科学2025，Vol.39Issue(3)：343-351,9.DOI:10.16819/j.1001-7216.2025.240509
水稻OsDR8基因的稻瘟病抗性评价及优异单倍型鉴定
Evaluation of Blast Resistance and Identification of Superior Haplotype of OsDR8 in Rice
摘要
Abstract
[Objective]Rice(Oryza sativa L.)is a crucial staple crop globally.Its production is severely threatened by rice blast disease.Identifying resistance materials and analyzing resistance genes are essential for understanding the mechanisms behind rice blast resistance.This study aims to systematically generate and identify genetic materials of the rice blast resistance gene OsDR8,conduct haplotype and evolutionary analyses to select superior haplotypes,and investigate their subpopulation differentiation.These efforts will lay a theoretical basis for the application of OsDR8 in rice blast resistance breeding programs.[Method]We developed transgenic lines overexpressing the OsDR8 gene and its knockout mutants.We conducted rice blast resistance assays on the overexpression lines,homozygous knockout lines,and corresponding wild-type materials using Magnaporthe oryzae isolates RO1-1 and RB22.Meanwhile,haplotype and evolutionary analyses of the OsDR8 gene were conducted based on the rice super pan-genome variation map.[Result]Disease symptom observation and disease resistance evaluation indicated that knockout mutants had significantly larger lesion sizes and a higher proportion of diseased leaf area compared to the wild-type.In contrast,the overexpression lines showed reduced lesion sizes and a lower proportion of diseased leaf area.Combined with haplotype and evolutionary analyses,the results indicated that materials of the superior haplotype Hap2 show stronger disease resistance,with 95.52%of the indica rice subpopulation being the Hap2 haplotype.[Conclusion]The OsDR8 gene positively regulates resistance to rice blast,and that the superior haplotype Hap2 may be associated with stronger resistance in indica rice.关键词
水稻/稻瘟病/优异单倍型/育种应用Key words
rice/rice blast/superior haplotype/breeding application引用本文复制引用
张彬涛,刘聪聪,郭明亮,杨绍华,吴世强,郭龙彪,朱义旺..水稻OsDR8基因的稻瘟病抗性评价及优异单倍型鉴定[J].中国水稻科学,2025,39(3):343-351,9.基金项目
国家重点研发计划资助项目(2022YFE0139400) （2022YFE0139400）
深圳市科技计划资助项目(GJHZ20190821163601707) （GJHZ20190821163601707）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021002). （XTCXGC2021002）
