中国水稻科学2025，Vol.39Issue(3)：365-372,8.DOI:10.16819/j.1001-7216.2025.240104
广谱抗稻瘟病基因PigmR的KASP标记开发及应用
Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast
摘要
Abstract
[Objective]The broad-spectrum resistance gene PigmR has important breeding value for the genetic improvement of rice blast resistance.Developing kompetitive allele specific PCR(KASP)markers based on its coding region variation sites is beneficial for improving the accuracy and efficiency of PigmR utilization in breeding.[Method]This study identified single nucleotide variations in the coding region of PigmR through sequence alignment,developed the KASP molecular marker PigmR-K,conducted genotype analysis using rice varieties and F2 segregating populations,combined these results with rice blast symptom identification,and carried out molecular marker assisted selection breeding to verify the precision and efficiency of this marker.[Result]Based on an A-to-C mutation at position 2503 in the PigmR gene coding region sequence,a precise and efficient PigmR genotyping technology system based on KASP markers was established.The genotyping of 159 rice varieties,270 F2 individuals,and 247 BC1F3 population lines demonstrated that PigmR-K reliably distinguished the homozygous resistant,homozygous susceptible and heterozygous genotypes of PigmR.These results were completely consistent with disease symptoms from artificial panicle blast inoculation.[Conclusion]The KASP marker PigmR-K developed based on the variation site of on the coding region has advantages such as precision and efficiency,which is beneficial for genetic improvement of rice blast resistance.关键词
水稻/广谱抗性/稻瘟病/PigmR基因/KASP/育种效率Key words
rice/broad-spectrum resistance/rice blast/PigmR gene/KASP/breeding efficiency引用本文复制引用
李文奇,杨杰,许扬,王芳权,朱建平,陶亚军,李霞,范方军,蒋彦婕,陈智慧..广谱抗稻瘟病基因PigmR的KASP标记开发及应用[J].中国水稻科学,2025,39(3):365-372,8.基金项目
江苏省种业振兴揭榜挂帅项目[JBGS(2021)039] （2021）
江苏省重点研发计划(现代农业)资助项目(BE2021374) （现代农业）
国家自然科学基金面上项目(42071023) （42071023）
生物育种钟山实验室项目(BM2022008-03) （BM2022008-03）
江苏省农业科学院基本科研业务专项(ZX(21)1201) （ZX(21）
江苏省农业科技自主创新资金资助项目(CX(19)1002) （CX(19）
江苏省自然科学基金资助项目(BK20212010). （BK20212010）
