中国输血杂志2025，Vol.38Issue(5)：605-609,5.DOI:10.13303/j.cjbt.issn.1004-549x.2025.05.002
脐血造血干细胞体外培养巨核系的CD36基因表达研究
Study on CD36 gene expression in megakaryocytic lineage derived from umbilical cord blood hematopoietic stem cells cultured in vitro
摘要
Abstract
Objective To elucidate the expression level and transcript structure of the CD36 gene in megakaryocytes cultured in vitro.Methods Using umbilical cord blood CD34+hematopoietic stem cells as the starting point,megakaryo-cytic lineage cells were directionally cultured in vitro using different combinations of cytokines.Total cell RNA was extracted from cultures at 0 d,7 d,and 12 d,and megakaryocyte RNA was extracted from CD41a-sorted cells after 14 d and 18 d of culture.RNA-NGS sequencing technology was used to analyze the RNA gene expression profiles across the five culture peri-ods and further investigate CD36 gene expression.Results The number of megakaryocytes generated in the TPO(100 ng/mL)group[(2.2±0.02)×106/mL]was significantly higher than that in the other five groups.A total of 22 066 expressed genes were detected in the RNA of the five culture periods,and gene expression levels at each time point were correlated with the culture timeline.CD36 gene expression increased with culture time,with FPKM values for CD36 expression in the megakaryocytic lineage at 14 d and 18 d being 18.35 and 101.85,respectively,which were much lower than those for IT-GA2B and ITGB3 genes but slightly higher than that for CD109 gene in the megakaryocytic lineage.The longest transcript of CD36 in the 18 d megakaryocytic lineage was 3.8 kb,encompassing all sequence of exons E3 to E14 and a partial sequence of E15.Conclusion This is the first report on the expression level and transcript structure of the CD36 gene in megakaryo-cytes cultured in vitro,providing fundamental data for research on the expression and regulation of the CD36 gene in the megakaryocytic lineage.关键词
CD36/巨核系/血小板/基因表达Key words
CD36/megakaryocytic lineage/platelets/gene expression分类
医药卫生引用本文复制引用
徐芳,俞广舒,凌霞,何吉,许先国..脐血造血干细胞体外培养巨核系的CD36基因表达研究[J].中国输血杂志,2025,38(5):605-609,5.基金项目
中国输血协会威高科研基金重点项目(CSBT-MWG-2023-01) （CSBT-MWG-2023-01）
