中国输血杂志2025，Vol.38Issue(5)：665-672,8.DOI:10.13303/j.cjbt.issn.1004-549x.2025.05.011
流式检测ABO抗原表达量的新方法
A new method for flow cytometry-based detection of ABO antigen expression levels
摘要
Abstract
Objective To design and establish a new method for flow cytometry-based detection of commonly observed highly expressed antigens on red blood cells,and to further evaluate the differences and distribution characteristics of antigen expression levels between ABO blood type homozygotes and heterozygotes in healthy individuals.Methods Residual blood samples after donor blood type identification by Shanghai Blood Center in April 2024 were collected.Among them,samples of 19 homozygous and 19 heterozygous individuals of type A and type B were selected.Then the expression level of ABO an-tigen on red blood cells were detected using the new method established in this study and the traditional aldehyde fixed red blood cell method.Both methods were tested independently three times and the results were compared.Results The mean values of the three detection results of the new method was(×105/RBC):AA homozygous 3.3±0.5,AO heterozygous 2.8±0.3,BB homozygous 3.6±0.3,BO heterozygous 3.1±2.8.The mean values of the three detection results of the aldehyde fixation method were AA homozygous 5.9±0.9,AO heterozygous 5.0±1.4,BB homozygous 3.8±0.6,and BO heterozy-gous 3.3±0.4.The average antigen distribution of each genotype followed a normal distribution.Comparing the average an-tigen expression levels of homozygotes and heterozygotes,both methods showed that A/B homozygotes had higher antigen levels than heterozygotes,with AA being 1.17 to 1.18 times that of AO and BB being 1.15 to 1.16 times that of BO.Com-paring the inter batch differences in the three test results of two methods,the new method showed no significant difference in the three test results for four genotypes(P>0.05).The aldehyde fixation method showed significant differences in the test results for all three genotypes(P<0.01)except for BB homozygotes(P>0.05).The reliability and reproducibility of the new method were better than those of the traditional aldehyde fixation method.Conclusion The antigen expression level of ABO homozygotes is higher than that of heterozygotes,and the difference in antigen level between type A homozygotes and heterozygotes is slightly higher than that of type B.The new method is superior to traditional aldolization fixation methods.关键词
ABO血型系统/IgM抗体/二硫苏糖醇/流式细胞术Key words
ABO blood-group system/IgM/dithiothreitol/flow cytometry分类
医药卫生引用本文复制引用
张玉宇,刘曦,谢军华,曹斌,郑皆炜,朱心怡,王中英,向东..流式检测ABO抗原表达量的新方法[J].中国输血杂志,2025,38(5):665-672,8.基金项目
中国输血协会圣湘输血医学发展基金(CSBT-SX-2024-06) （CSBT-SX-2024-06）
