中国输血杂志2025，Vol.38Issue(5)：673-677,5.DOI:10.13303/j.cjbt.issn.1004-549x.2025.05.012
短波紫外线灭活血管性血友病因子制品中病毒的研究
Viral inactivation in von Willebrand factor preparations via UVC irradiation:an experimental approach
摘要
Abstract
Objective To evaluate the efficacy of ultraviolet-C(UVC)irradiation in inactivating porcine parvovirus(PPV),encephalomyocarditis virus(EMCV),pseudorabies virus(PRV)and vesicular stomatitis virus(VSV)within von Willebrand factor(vWF)preparations.Methods An ultraviolet inactivator(254 nm primary wavelength)was employed to optimize UV exposure dosages for the samples,and the initial test groups were set at five irradiation gradients:100,200,400,1 000,and 1 500 J/m2 gradients.Based on the results of the preliminary experiments,subsequent formal experiments implemented refined dosage parameters at 100,125,150,175,and 200 J/m2.Virucidal efficacy against various pathogen-ic strains was evaluated via cytopathic effect(CPE)observation methodology,while simultaneously quantifying von Wille-brand factor antigen levels(vWF∶Ag)and collagen-binding activity(vWF∶CBA)using enzyme-linked immunosorbent as-says(ELISA)and automated coagulation analysis systems before and after UVC treatment.Results The results showed that>100 J/m2 UVC irradiation achieved a≥4.0 log reduction factor in vWF.For vWF antigen,retention rates were ob-served to be 93.67%,91.72%,93.54%,79.05%,and 85.50%at UVC doses of 100,125,150,175,and 200 J/m2,respectively.Similarly,the retention rates of vWF collagen-binding activity were 81.11%,87.81%,86.15%,73.73%,and 73.72%under the same dose conditions.Conclusion Considering both inactivation efficacy and functional preserva-tion while ensuring safety and effectiveness,100-150 J/m2 represents the optimal inactivation dose.关键词
短波紫外线/血管性血友病因子/病毒灭活/猪细小病毒/伪狂犬病毒/脑心肌炎病毒/水疱性口炎病毒Key words
UVC/vWF/virus inactivation/PPV/PRV/EMCV/VSV分类
医药卫生引用本文复制引用
张雅路,马杰,张容,杨春晖..短波紫外线灭活血管性血友病因子制品中病毒的研究[J].中国输血杂志,2025,38(5):673-677,5.基金项目
中国医学科学院创新项目工程(2021-I2M-1-060) （2021-I2M-1-060）
