中国兽医科学2025，Vol.55Issue(5)：586-591,6.DOI:10.16656/j.issn.1673-4696.2025.0071
绵羊肺炎支原体毒力评价细胞模型的建立
Establishment of a cell model for evaluating the virulence of Mycoplasma ovipneumoniae
摘要
Abstract
To establish a cell model for virulence evaluation of the Mycoplasma ovipneumoniae by detecting and analyzing the virulence-related factors of cells infected with M.ovipneumoniae.In this study,5 M.ovipneumoniae strains with known virulence of 108 CCU50/mL were employed to infect the MDBK cell.IFA and quantitative PCR method were used to detect the adhesion ability of M.ovipneumoniae strains.Cytopathogenic effect(CPE)was observed.And cell viability,cytotoxicity,and intracellular H2O2 content were measured by using the commercial kits.According to the results of significance analysis,the virulence of 5 M.ovipneumoniae strains was evaluated.Base on the results of correlation analysis,the most relevant virulence evaluation factors of M.ovipneumoniae strain were screened.The adhesion ability,damage effect on cell activity,damage effect on cell membrane integrity,and mediating effect of IK3-3 and NJ01 were stronger than those of Y98,N3-2F and XLY.The results indicated that IK3-3 and NJ01 were highly virulent strains,N3-2F and XLY were moderately virulent strains,and Y98 was a weak virulence strain,which was consistent with the results of the previous animal infection test.The results of correlation analysis showed that the virulence evaluation factors of the cell infection virulence evaluation model were feasible,and the adhesion ability of M.ovipneumoniae was the most correlated factor with its virulence.The cell model established in this study provides a new test platform for evaluating virulence,studying the pathogenic mechanism and screening vaccine candidates of M.ovipneumoniae.关键词
绵羊肺炎支原体/毒力/细胞感染Key words
Mycoplasma ovipneumoniae/virulence/cell infection分类
农业科技引用本文复制引用
单依依,刘茂军,刘娜,程子龙,陈益,王晓楠,张纹纹,李文良,杨蕾蕾,冯志新..绵羊肺炎支原体毒力评价细胞模型的建立[J].中国兽医科学,2025,55(5):586-591,6.基金项目
国家自然科学基金青年基金项目(32202809) （32202809）
江苏省农业科学院探索性颠覆性项目[ZX(21)1218] （21）
动物疫病防控全国重点实验室开放基金项目(SKLVEB-KFKT-04) （SKLVEB-KFKT-04）
