中国兽医科学2025，Vol.55Issue(5)：632-639,8.DOI:10.16656/j.issn.1673-4696.2025.0064
凝集素Q-Griffithsin的克隆表达及其对猪流行性腹泻病毒的抑制作用
Cloning and expression of agglutinin Q-Griffithsin and its antiviral activity against porcine epidemic diarrhea virus
摘要
Abstract
To investigate the inhibitory effect of the antioxidant derivative Q-Griffithsin(Q-GRFT)on porcine epidemic diarrhea virus(PEDV),a recombinant expression plasmid pGEX-4T-Q-GRFT was constructed,and the recombinant protein Q-GRFT was expressed using a prokaryotic expression system.The target protein was purified by nickel-affinity chromatography and its specificity of the concen-trated protein was validated.Its anti-PEDV effect was further confirmed by in vitro experiments.The results showed that the pGEX-4T-Q-GRFT was successfully transformed into competent cells.Following induction at 22 ℃ for 14 h,the Q-GRFT protein was expressed at its anticipated molecular weight,with a purification yield of 3.01 mg/L.In vitro assay,Q-GRFT markedly mitigated the cytopathic effects induced by PEDV infection,inhibited PEDV replication within cells,and demonstrated antiviral activity in a mass concentration-dependent.In summary,this study successfully prepared a recombinant protein Q-GRFT possessing anti-PEDV activity,providing a theoretical foundation for the development of antiviral agents against animal coronaviruses,and proposing innovative strategies for PEDV prophylaxis and treatment.关键词
Q-Griffithsin/猪流行性腹泻病毒/蛋白表达/抗病毒活性Key words
Q-Griffithsin/porcine epidemic diarrhea virus/protein expression/antiviral activity分类
农业科技引用本文复制引用
周炫汝,刘子岚,林起鑫,梅朋,蔡淑铭,姜文亿,吴异健,刘萍..凝集素Q-Griffithsin的克隆表达及其对猪流行性腹泻病毒的抑制作用[J].中国兽医科学,2025,55(5):632-639,8.基金项目
福建省自然科学基金项目(2021J01082) （2021J01082）
福建省教育厅中青年教师教育科研项目(JAT200092,JAT200099) （JAT200092,JAT200099）
