中国兽医科学2025，Vol.55Issue(5)：645-654,10.DOI:10.16656/j.issn.1673-4696.2025.0068
甘肃一起小反刍兽疫病例中流行毒株的生物学特性和遗传进化分析
Characterization and evolutionary analysis of the prevalent strain in a peste des petits ruminants case in Gansu Province
摘要
Abstract
A case of peste des petits ruminants(PPR)caused lamb death was found in a sheep farm in Linxia,Gansu Province,in November 2023.The disease was diagnosed by clinical symptoms,pathological autopsy observation and real-time RT-PCR detection.The whole genome sequence of the peste des petits ruminants virus(PPRV)epidemic strain was amplified by RT-PCR.Genetic analysis was carried out based on the whole genome,F gene,H gene and Ngene sequences obtained in this study and reference sequences from GenBank.The effects of PPRV vaccine strain on the proliferation and differentiation of sheep peripheral blood mononuclear cells(PBMCs)were investigated by CCK-8 reagent and flow cytometry.The results showed that the epidemic strain China/GS/2023 responsible for the PPR case belonged to genotype Ⅳ,with a total length of 15 954 bp.Phylogenetic analysis showed that the epidemic strain was closely related to China/XJYL/2013,China/3/2013,and China/GS/2018.In addition,different titers of live-attenuated PPRV vaccine strain Nigeria75/1 could inhibit the proliferation of sheep PBMCs in vitro,while high titers of PPRV vaccine strain Nigeria75/1 inhibited the differentiation of CD4+and CD8+in T lymphocytes.These results suggest that the infection of China/GS/2023 may be originated from the 2013-2014 PPR epidemic.In addition,application of the PPRV vaccine incorrectly including repeated immunization and over-dose of immunization may also be the causes of re-infection and outbreak of PPR cases in China.关键词
小反刍兽疫病毒/病理剖检/遗传进化分析/流行病学Key words
peste des petits ruminants virus/pathological necropsy/genetic analysis/epidemiology分类
农业科技引用本文复制引用
刘萍,杜国玉,柳周斌,聂帅帅,吴锦艳,曹小安,何继军,尚佑军..甘肃一起小反刍兽疫病例中流行毒株的生物学特性和遗传进化分析[J].中国兽医科学,2025,55(5):645-654,10.基金项目
国家现代农业产业技术体系专项(CARS-39-04B) （CARS-39-04B）
国家绒毛用羊产业技术体系专项(CARS-39-13) （CARS-39-13）
甘肃省科技特派团专项(22CX8NA012) （22CX8NA012）
国家重点研发计划项目(2021YFD1800504,2022YFD1602203,2022YFD1302101) （2021YFD1800504,2022YFD1602203,2022YFD1302101）
