中国兽医科学2025，Vol.55Issue(5)：669-677,9.DOI:10.16656/j.issn.1673-4696.2025.0086
SUN2调控稳定表达绵羊肺腺瘤病毒囊膜蛋白的OAR-L1细胞的恶性转化能力的研究
Study of the ability of SUN2 to regulate the malignant transformation of OAR-L1 cells stably expressing the envelope protein of jaagsiekte sheep retrovirus
摘要
Abstract
To explore the function of SAD1/UNC84 domain protein-2(SUN2)in the malignant transforma-tion of tumor cells in ovine pulmonary adenocarcinoma(OPA).Sheep lung fibroblasts(OAR-L1)stored in the laboratory were infected with JSRV env lentivirus,resulting in the successful establishment of a stable OAR-L1-Env cell strain.Expressions of JSRV Env and SUN2 were detected through qPCR,and Western-blot.The malignant transformation ability of cells was determined by CCK-8,wound healing assay,and soft agarose colony formation assay.Additionally,SUN2 overexpression and silencing plasmids were constructed and transfected into OAR-L1-Env stable cell strain,and expression of SUN2 in the OAR-L1-Env stable cell strain was detected by qPCR and Western-blot.Proliferation and migration of cells were examined by CCK-8 and wound healing assays.The results demonstrated that the OAR-L1-Env stable cell strain was successfully established,and expression of SUN2 was significantly downregulated(P＜O.05).CCK-8,wound healing assay and soft agarose colony formation assay indicated the malignant transformation of OAR-L1-Env stable cell strain.Overexpression of SUN2 in the OAR-L1-Env stable cell strain inhibited cell proliferation and mi-gration(P＜0.05),while silencing SUN2 promoted cell proliferation and migration(P＜0.05).In conclu-sion,this study suggested that SUN2 is involved in the regulation of JSRV env-induced cell malignant transformation,suggesting that SUN2 may have a positive inhibitory effect on OPA tumorigenesis.This study provides some reference materials for further investigation on the biological role of SUN2 in cell proliferation in OPA.关键词
稳转株/囊膜蛋白/SUN2/增殖/迁移Key words
stable cell line/envelope protein/SUN2/proliferation/migration分类
农业科技引用本文复制引用
鲍安玉,杜晓悦,陈思旭,段续接,张培,刘淑英..SUN2调控稳定表达绵羊肺腺瘤病毒囊膜蛋白的OAR-L1细胞的恶性转化能力的研究[J].中国兽医科学,2025,55(5):669-677,9.基金项目
国家自然科学基金项目(32072819,32360863) （32072819,32360863）
内蒙古科技重大专项(2021ZD0010) （2021ZD0010）
内蒙古草原英才创新团队项目(20151031) （20151031）
内蒙古自治区教育厅高校创新团队建设项目(BR22-13-08) （BR22-13-08）
兽医基础与牛羊疫病防控技术研发创新团队项目(NMGIRT2412) （NMGIRT2412）
内蒙古自治区教育厅一流学科科研专项(YLXKZX-NND-012) （YLXKZX-NND-012）
