中国兽医科学2025，Vol.55Issue(5)：678-686,9.DOI:10.16656/j.issn.1673-4696.2025.0085
基于体外试验和网络药理学探究千金藤素抗IBV作用机制
Network pharmacology and in vitro experimental validation to reveal the mechanism of cepharanthine against IBV
摘要
Abstract
This study aimed to explore the effects,potential active ingredients and mechanism of cepharanthine(CEP)against infectious bronchitis virus(IBV)based on in vitro experiments and network pharmacology.Firstly,the CEP was prepared and its cytotoxicity on Vero cells was determined,and then the inhibitory effects of the CEP on IBV proliferation at different times and doses were determined by RT-qPCR,Western-blot and indirect immunofluorescence assay.Secondly,the active ingredients and targets of the CEP were collected from the Swiss target prediction and Targetnet databases,and the IBV diseases targets were collected from the GeneCards databases,then their intersection targets were obtained and the corresponding protein-protein interaction network was constructed.Furthermore,GO function analysis and KEGG pathway enrichment analysis were conducted.The results of in vitro experiments showed that the maximum cell safety concentration of the CEP significantly inhibited the virus proliferation at 48 hours post IBV infection,and the low,medium and high concentrations of the CEP could inhibit IBV proliferation in a dose-dependent manner.The results of network pharmacology showed that there are 88 active ingredi-ents in the CEP,which can act on 46 IBV disease targets.GO function analysis yielded 122 entries,and KEGG pathway enrichment analysis yielded 147 signaling pathways.This research results indicated that the CEP has direct anti-IBV effect and may exert indirect anti-IBV effect by acting on targets such as AKT1,EGFR,SRC,MTOR,PIK3CA and ERBB2.关键词
千金藤素/禽传染性支气管炎病毒/Vero细胞/网络药理学Key words
cepharanthine/infectious bronchitis virus/Vero cell/network pharmacology分类
农业科技引用本文复制引用
许秋池,李旭,郭津玮,苑金利,陈杉,侯晓林..基于体外试验和网络药理学探究千金藤素抗IBV作用机制[J].中国兽医科学,2025,55(5):678-686,9.基金项目
国家自然科学基金项目(31372485) （31372485）
