基于体外试验和网络药理学探究千金藤素抗IBV作用机制OA北大核心

This study aimed to explore the effects,potential active ingredients and mechanism of cepharanthine(CEP)against infectious bronchitis virus(IBV)based on in vitro experiments and network pharmacology.Firstly,the CEP was prepared and its cytotoxicity on Vero cells was determined,and then the inhibitory effects of the CEP on IBV proliferation at different times and doses were determined by RT-qPCR,Western-blot and indirect immunofluorescence assay.Secondly,the active ingredients and targets of the CEP were collected from the Swiss target prediction and Targetnet databases,and the IBV diseases targets were collected from the GeneCards databases,then their intersection targets were obtained and the corresponding protein-protein interaction network was constructed.Furthermore,GO function analysis and KEGG pathway enrichment analysis were conducted.The results of in vitro experiments showed that the maximum cell safety concentration of the CEP significantly inhibited the virus proliferation at 48 hours post IBV infection,and the low,medium and high concentrations of the CEP could inhibit IBV proliferation in a dose-dependent manner.The results of network pharmacology showed that there are 88 active ingredi-ents in the CEP,which can act on 46 IBV disease targets.GO function analysis yielded 122 entries,and KEGG pathway enrichment analysis yielded 147 signaling pathways.This research results indicated that the CEP has direct anti-IBV effect and may exert indirect anti-IBV effect by acting on targets such as AKT1,EGFR,SRC,MTOR,PIK3CA and ERBB2.