中国兽医杂志2025，Vol.61Issue(5)：65-72,8.DOI:10.20157/j.cnki.zgsyzz.2025.05.008
基于DIA技术的多药耐药性捻转血矛线虫蛋白质组学分析
Proteomic Analysis of Multidrug Resistance of Haemonchus contortus by DIA Technology
摘要
Abstract
To explore the mechanisms underlying the development of anthelmintic resistance,this study treated Haemonchus contortus third stage larvae(L3)with ivermectin,albendazole,and levamisole,and employed data-independent acquisition(DIA)proteomic sequencing to analyze the differential protein expression before and after drug treatment,to identify multidrug resistance-related proteins.The results revealed a total of 1 888 differentially expressed proteins,of which 63 were related to multidrug resistance.Further analysis revealed 36 proteins with significantly different expression(P<0.05),of which 25 were specific to Haemonchus contortus and related to drug resistance.Gene ontology(GO)analysis classified 60 of these differential proteins into 76 biological process branches,while Kyoto encyclopedia of genes and genomes(KEGG)analysis revealed that 46 differentially expressed proteins were enriched in 75 signaling pathways related to lipid metabolism and endocrine systems.Comprehensive analysis identified five Haemonchus contortus specific drug resistance proteins with significant expression differences:proteins containing UDP-glucuronosyltransferase domains,antigen H11,immunoglobulin v and i groups,proteins with protein tyrosine kinase(PTK)domains,and proteins with the lethal giant larvae homolog 2 domain.These proteins are involved in important biological processes and key metabolic pathways.The results of this study provide foundational data for understanding the relationship between differentially expressed proteins and lipid metabolism,endocrine systems,and multidrug resistance in Haemonchus contortus,and have significant implications for revealing the mechanisms underlying multidrug resistance in this parasitic nematode.关键词
多药耐药性/捻转血矛线虫/数据非依赖性采集(DIA)/蛋白质组学/耐药性相关蛋白质Key words
multidrug resistance/Haemonchus contortus/data-independent acquisition(DIA)/proteomics/resistance-re-lated protein分类
畜牧业引用本文复制引用
李炎夫,郭志凯,丁玉林,陆静,王瑞,贾晓晴,马梅清,杜山,焦万明,马园,郝陆瑶,张艳妮,李倩楠..基于DIA技术的多药耐药性捻转血矛线虫蛋白质组学分析[J].中国兽医杂志,2025,61(5):65-72,8.基金项目
内蒙古自治区重点研发和成果转化计划项目(2023YFDZ0048) （2023YFDZ0048）
内蒙古自然科学基金项目(2023LHMS03022,2023LHMS03005) （2023LHMS03022,2023LHMS03005）
鄂尔多斯市国家可持续发展议程创新示范区建设(含厅市会商、科技突围工程)科技支撑项目(KCX2024016) （含厅市会商、科技突围工程）
国家自然科学基金项目(32160838) （32160838）
国家乳业技术创新中心项目(2023-JSGG-5) （2023-JSGG-5）
