中国听力语言康复科学杂志2025，Vol.23Issue(3)：251-255,5.DOI:10.3969/j.issn.1672-4933.2025.03.007
经圆窗抽取后再注射地塞米松分布及安全性研究
Drug Distribution and Safety of Dexamethasone Injection after Extracting Perilympha through Round Window
摘要
Abstract
Objective Study of the maximum safe volume for lymphatic fluid extraction from the inner ear of guinea pigs,safety and efficacy of the procedure of extracting perilympha and then injecting drugs through a round window membrane(RWM).Methods Fifteen normal guinea pigs were taken and randomized into groups,and the maximum safe volume of outer lymphatic fluid to be extracted was determined by gradient extraction of outer lymphatic fluid from the inner ear followed by audiometry.Afterwards,20 normal guinea pigs were randomly divided into 3 groups:a blank control group,a systemic drug administration group,and an extracting perilympha and then injecting drugs via RWM group(group injecting the same volume of dexamethasone after via RWM extracting perilympha with the maximum safe extraction volume).(1)Efficacy experiment:the drug distribution efficiency was determined by comparing the fluorescence staining expression of the drug in the inner ear 2h after treatment.(2)Safety experiment:the safety of the operation was determined by comparing the mean thresholds of each frequency band of ABR 24 h after treatment.Results The maximum safe dose for guinea pig lymphatic fluid extraction through the inner ear was 4 μL.Dexamethasone was more efficiently distributed in the group of extracting perilympha and then injecting drugs via RWM.The mean ABR thresholds of the 24h postoperative in the extracting perilympha and then injecting drugs via RWM group had a short-term rise only at 32 kHz and did not affect the hearing thresholds at other frequencies.Conclusion The method of extracting perilympha and then injecting drugs via RWM can realize the efficient distribution of inner ear drugs with short-term damage to high-frequency hearing only.关键词
内耳/圆窗注射/外淋巴液/听性脑干反应Key words
Inner ear/Round window injection/Perilympha/Auditory brainstem response分类
临床医学引用本文复制引用
石岩,任巍,李雨青,马靖,曹大山,赵辉..经圆窗抽取后再注射地塞米松分布及安全性研究[J].中国听力语言康复科学杂志,2025,23(3):251-255,5.基金项目
国家重点研发计划项目"机器人辅助内耳注射采样手术评估方法与综合解决方案"(2023YFB4705804) （2023YFB4705804）
