中国药房2025，Vol.36Issue(10)：1179-1185,7.DOI:10.6039/j.issn.1001-0408.2025.10.05
右美托咪定增加分泌型SERPINE1表达对甲状腺癌细胞恶性生物学行为的影响
Effects of increased secretory SERPINE1 expression by dexmedetomidine on the malignant biological behavior of thyroid carcinoma cells
摘要
Abstract
OBJECTIVE To explore the effects of dexmedetomidine(DEX)increasing serpin peptidase inhibitor clade E member 1(SERPINE1)protein on the malignant biological behavior of thyroid carcinoma(THCA)cells.METHODS THCA cells(KTC-1,TPC-1)were treated with 1,10 and 100 nmol/L DEX,and their viabilities,clone formation rates,migration rates and invasion number were examined.Potential biological functions of DEX in THCA cells were analyzed through whole genome sequencing and gene ontology enrichment analysis.The core targets of DEX were mined through a protein-protein interaction network.The expression characteristics of DEX core targets and their relationship with patient prognosis were evaluated.The effects of DEX on mRNA and protein expressions of core targets and protein secretion in 2 types of THCA cells were detected,and the effects of this target on DEX-related effects were validated preliminarily by knocking down the core target.RESULTS Compared with the control group(0 nmol/L DEX),DEX at 1,10 and 100 nmol/L significantly increased the viabilities of 2 types of THCA cells(except for the KTC-1 cells in the 1 nmol/L DEX group at 24 h),concentration-dependently elevated the rates of clone formation,migration rates(except for 2 types of THCA cells in 1 nmol/L DEX group),and the number of invasion(P＜0.05).A total of 287 differently expressed genes(75 up-regulated and 212 down-regulated)were enriched in signaling pathways such as phosphatidylinositol 3-kinase/protein kinase B,Wnt,and senescence-associated secretory phenotypes in the 2 kinds of DEX-treated or non-treated THCA cells.SERPINE1 was a core target of DEX for THCA,and its mRNA and protein expression in THCA tissues/cells were significantly elevated and associated with poor prognosis of the patients(P＜0.05).Compared with the control group,mRNA and protein expression of SERPINE1 was significantly up-regulated in 2 types of cells in the 1,10 and 100 nmol/L DEX groups,while the secretion of this protein in conditioned medium was also significantly increased,all of which showed concentration-dependence(P＜0.05).After knocking down SERPINE1,the promoting effects of DEX on the proliferation,colony formation,migration and invasion abilities of two types of THCA cells were significantly inhibited(P＜0.05).CONCLUSIONS DEX can promote the proliferation,migration and invasion of THCA cell,and the above effects may be associated with the expression of increased secretory SERPINE1 protein.关键词
右美托咪定/甲状腺癌/SERPINE1/恶性生物学行为Key words
dexmedetomidine/thyroid carcinoma/SERPINE1/malignant biological behavior分类
药学引用本文复制引用
童雪燕,蒋文锋,曾亮平,林燕..右美托咪定增加分泌型SERPINE1表达对甲状腺癌细胞恶性生物学行为的影响[J].中国药房,2025,36(10):1179-1185,7.基金项目
江西省卫生健康委科技计划项目(No.202212777,No.20197555) （No.202212777,No.20197555）
上饶市科技计划项目(No.2023CZDX82) （No.2023CZDX82）
