中国医科大学学报2025，Vol.54Issue(5)：390-395,6.DOI:10.12007/j.issn.0258-4646.2025.05.002
基于非靶向代谢组学探讨龟鹿二仙胶防治绝经后骨质疏松症的机制
Mechanism of Guilu erxian glue in the treatment of postmenopausal osteoporosis based on non-targeted metabolomics
摘要
Abstract
Objective To explore the biological effects and changes of metabolites of Guilu erxian glue(GLE)in vivo in the pre-vention and treatment of postmenopausal osteoporosis(PMO),based on non-targeted metabolomics.Methods Fifty SD female rats were divided into five groups using the random number table method:sham group,model group(OVX),and low-,medium-,and high-dose GLE(GLE-L,GLE-M and GLE-H)groups,with ten mice in each group.After 1 week of modeling,the drug was given by gavage,and samples were collected 8 weeks later.Bone microstructure was detected by MicroCT,bone metabolism-related indices were analyzed by immunohistochemistry(IHC),and a serum metabolite atlas was constructed using the LC-MS/MS technique to observe the distribution differences of metabolites and related pathways.Results The MicroCT results showed that GLE could increase the percentage of bone trabeculae and restore bone microstructure(P<0.05,GLE-M group vs.OVX group).IHC analysis showed that GLE could increase Runx2 expression in femur(P<0.05,GLE-M group vs.OVX group).There were four co-regulated metabolites in the sham group,GLE-H group,and OVX group,including 3-(3-methoxyphenyl)propionic acid,acetamide,Equol,and 1-oleoacyl-SN-glycerol-3-phosphocholine,which showed high expression in the sham group,low expression in the OVX group,and high expression in the GLE-M group.The KEGG pathway result showed that the main pathways involved between the sham group and OVX group were steroid hormone biosynthesis and cortisol synthesis and secretion.Tryptophan metabolism and phenylalanine metabolism were observed in the GLE group and OVX group.Conclusion GLE may prevent PMO by regulating metabolites in the body.关键词
龟鹿二仙胶/非靶向代谢组学/代谢产物/绝经后骨质疏松症Key words
Guilu erxian glue/non-targeted metabolomics/metabolite/postmenopausal osteoporosis分类
中医学引用本文复制引用
于冬冬,郭强,陈奎竹,顾海立,乔隆,乔野..基于非靶向代谢组学探讨龟鹿二仙胶防治绝经后骨质疏松症的机制[J].中国医科大学学报,2025,54(5):390-395,6.基金项目
国家自然科学基金(82374480) （82374480）
