中国药理学与毒理学杂志2025，Vol.39Issue(5)：370-379,10.DOI:10.3867/j.issn.1000-3002.2025.08309
眼刺激体外测试方法及分层测试策略研究进展
Research progress in in vitro test methods and tiered strategies for eye irritation
摘要
Abstract
Assessment of eye irritation potential is part of the international regulatory requirements for safety testing of chemicals.Because of increasing concerns about to animal welfare in recent years,the development of alternative methods to reduce the number of animals used in eye irritation tests has become imperative.This article introduces in vitro test methods based on organotypic models,cell lines and reconstructed human cornea-like epithelium(RhCE),and analyzes strengths and weaknesses of the individual in vitro test methods.To overcome the limitations of the individual in vitro test methods,it is recommended that tiered testing strategies be adopted that combine the strengths of individual in vitro test methods to address the required ranges of irritation potential.Future applications of new technolo-gies such as genomics,proteomics and computational models in the assessment of eye irritation poten-tial are also predicted.关键词
眼刺激/体外测试方法/分层测试策略Key words
eye irritation/in vitro test methods/tiered testing strategies分类
药学引用本文复制引用
鲍佳生,李梦圆,黄丽霞,潘丙珍,周智明..眼刺激体外测试方法及分层测试策略研究进展[J].中国药理学与毒理学杂志,2025,39(5):370-379,10.基金项目
广州海关科研项目(2022GZCK06) （2022GZCK06）
广州海关科研项目(2023GZCK07) （2023GZCK07）
广东省药品监督管理局化妆品风险评估重点实验室专项(2023ZDZ11)Guangzhou Customs Scientific Research Project(2022GZCK06) （2023ZDZ11）
Guangzhou Customs Scientific Research Project(2023GZCK07) （2023GZCK07）
and GDMPA Project of Key Laboratory for Cosmetics Risk Assessment(2023ZDZ11) （2023ZDZ11）
