中国药物经济学2025，Vol.20Issue(4)：108-111,4.DOI:10.12010/j.issn.1673-5846.2025.04.023
HS-SPME-GC-MS联用分析都梁丸中挥发性成分
Analysis of Volatile Components in Duliang Pills by HS-SPME-GC-MS
摘要
Abstract
Objective Determine the volatile components in Duliang Pills.Methods The samples were analyzed using headspace-solid phase microextraction(HS-SPME)coupled with gas chromatography-mass spectrometry(GC-MS)instrument.GC-MS conditions were as follows:HP-5MS5%Phenyl Methyl Silox column(30 m×250 mm,0.25 µm),initial temperature of 50℃,and heating at 5℃/min to 230℃.The inlet temperature is 250℃,the carrier gas is 99.99%He,and the carrier gas flow rate is 1 ml/min;No diversion injection.The energy of the EI ionization source is 70 eV,and the temperature of the ion source is 230℃;Quadrupole rod temperature 150℃;Doubling tube voltage 1.2 kV;The scanning quality range is 35～550 m/z.Conditions for headspace microextraction:Weigh approximately 3.0 g of sample powder accurately,place it in a 15 ml headspace bottle,insert a manual sampler containing a 65 μm PDMS/DVB extraction fiber head,pre equilibrate at 80℃for 10 minutes,extract for 30 minutes,and immediately insert it into the chromatograph injection port(temperature 230℃);Desorption for 5 minutes.Results 87 components were detected in the powder of Duliang Pills,and 46 components were identified,accounting for 52.87%of the total volatile components.Conclusion This determination method can quickly and accurately determine and identify the volatile components in Duliang Pills,providing a scientific basis for the analysis of the pharmacological substance basis of Duliang Pills.关键词
都梁丸/挥发性成分/顶空进样/气相色谱-质谱联用法Key words
Duliang Pills/Volatile components/Headspace injection/Gas chromatography-mass spectrometry分类
医药卫生引用本文复制引用
马立立,赵林超,高浩然,王静,袁子民..HS-SPME-GC-MS联用分析都梁丸中挥发性成分[J].中国药物经济学,2025,20(4):108-111,4.基金项目
辽宁省教育厅2022年度科学研究经费研究项目(LJKMZ20221320) （LJKMZ20221320）
2024年度辽宁省教育厅基本科研项目储备项目(LJKMZ2024001) （LJKMZ2024001）
辽宁省自然科学基金资助项目(2019-ZD-0440) （2019-ZD-0440）
