中国药物评价2025，Vol.42Issue(2)：107-110,4.
HPLC法测定和中降浊调控丸中2种有效成分的含量
Determination of the Contents of Two Effective Components in Hezhong Jiangzhuo Control Pills by HPLC
摘要
Abstract
Objective:To develop a high-performance liquid chromatography(HPLC)analytical approach enabling the simultaneous determination of the contents of two active ingredients in Hezhong Jiangzhuo control pills,thus providing a reference for its quality control.Methods:An ACES C18 chromatographic column(250 mm ×4.6 mm,5 μm)was employed.The mobile phase consisted of acetonitrile and water for gradient elution.The flow rate was set at 1.0 mL·min-1,and the column temperature was maintained at 35 ℃.Dual-wavelength detection was performed at 283 nm and 340 nm,with an injection volume of 5 μL.Results:For atractylodin and hesperidin in Hezhong Jiangzhuo control pills,excellent linear relationships between their contents and peak areas were observed within the ranges of 0.0214 μg to 0.2672 μg and 0.0768 μg to 0.9597 μg respectively(r2≥0.999 9).The relative standard deviation(RSD)values ob-tained from the precision,stability,and repeatability tests were all less than 2.0％.The average recoveries of atractylodin and hesperidin were 99.64％and 101.7％respectively(n=6).In samples from three different packaging batches,the measured contents of atractylodin and hesperidin were 0.21 mg·g-1 and 1.27 mg·g-1,0.22 mg·g-1 and 1.19 mg·g-1,as well as 0.22 mg·g-1 and 1.23 mg·g-1 respectively.Conclusion:This method is relatively straightforward to operate and exhibits high sensitivity.The results are stable and relia-ble,making it suitable for the quality control of atractylodin and hesperidin in Hezhong Jiangzhuo control pills.关键词
和中降浊调控丸/橙皮苷/苍术素/含量测定Key words
Hezhong jiangzhuo control pills/Hesperidin/Atractylotin/Content determination分类
中医学引用本文复制引用
周丽霞,王瑞琪,杨娜,刘闪闪,张峰,李清芳..HPLC法测定和中降浊调控丸中2种有效成分的含量[J].中国药物评价,2025,42(2):107-110,4.基金项目
国家中医药管理局中医药科学研究专项课题(GZY-KJS-2021-018) （GZY-KJS-2021-018）
河南省中医药科学研究专项课题(20230317) （20230317）
