中国医学前沿杂志（电子版）2025，Vol.17Issue(4)：48-56,9.DOI:10.12037/YXQY.2025.04-08
基于单细胞测序解析小胶质细胞衰老在糖尿病脑病中的作用
Analysis of the role of microglia senescence in diabetic encephalopathy based on single-cell sequencing
摘要
Abstract
Objective To investigate the expression of celluar senescence-related genes in microglia of mice with diabetic encephalopathy.Methods Single-cell sequencing technique was used to map the hippocampus of diabetic encephalopathy mice.The expression of cellular senescence related genes in microglia was detected by qRT-PCR.Results The expression levels of cellular senescence related genes Calm2,Mapkapk2,Nfkb1,H2-D1,Ccnd3,Cdk6 and Il-1α were determined by single-cell mapping and dimensional clustering analysis of the hippocampus in diabetic encephalopathy.qRT-PCR showed that the mRNA expression levels of Mapkapk2,Nfkb1,H2-D1,Cdk6 and Il-1α were significantly increased in BV2 cells treated with high glucose,while the mRNA expression levels of Calm2 and Ccnd3 were significantly decreased.Conclusions Single-cell sequencing revealed that diabetic encephalopathy affects gene expression associated to microglial senescence,providing a molecular mechanism and potential therapeutic target for diabetic encephalopathy.关键词
糖尿病脑病/单细胞测序/小胶质细胞/细胞衰老Key words
Diabetic encephalopathy/Single-cell sequencing/Microglia/Cellular senescence引用本文复制引用
张婧语,黄晴,戴祺,周奕婷,莫蝶,付彰,林夏鸿..基于单细胞测序解析小胶质细胞衰老在糖尿病脑病中的作用[J].中国医学前沿杂志（电子版）,2025,17(4):48-56,9.基金项目
the National Natural Science Foundation of China(82270873) （82270873）
Natural Science Foundation of Shenzhen Municipality(JCYJ20240813150436048) （JCYJ20240813150436048）
Shenzhen Key Laboratory of Bone Tissue Repair and Translational Research(ZDSYS20230626091402006) 国家自然科学基金(82270873) （ZDSYS20230626091402006）
深圳市科学创新委员会基金(JCYJ20240813150436048) （JCYJ20240813150436048）
深圳骨组织修复和转化研究重点实验室(ZDSYS20230626091402006) （ZDSYS20230626091402006）
