内科2025，Vol.20Issue(1)：1-9,9.DOI:10.16121/j.cnki.cn45-1347/r.2025.01.01
黄根片对胶质瘤U87-MG细胞恶性生物学行为的影响及机制研究
Effects of Huanggen Tablets on the malignant biological behaviors of glioma U87-MG cells and its mechanisms
摘要
Abstract
Objective To explore the inhibitory effects of Huanggen Tablets on the proliferation,migration,and invasion of glioma U87-MG cells and the possible mechanisms.Methods U87-MG cells were intervened with Huanggen Tablets components solutions at different crude drug concentrations(0.125 mg/mL,0.25 mg/mL,0.5 mg/mL,1 mg/mL,2 mg/mL,4 mg/mL,8 mg/mL,16 mg/mL,32 mg/mL,64 mg/mL).The 50%inhibitory concentration(IC50)of the drug at 24 hours after the intervention was calculated according to the results of the CCK-8 assay,based on which the subsequent experiment was designed to set up a control group,as well as a low-dose Huanggen Tablets group,a medium-dose Huanggen Tablets group,and a high-dose Huanggen Tablets group(with crude Huanggen concentrations of 3.25 mg/mL,6.5 mg/mL,and 13 mg/mL,respectively).The CCK-8 assay and plate colony formation assay were used to detect the cell viability and relative cloning formation rate of U87-MG cells in each group.The Transwell migration and invasion assays were used to detect the relative migration and invasion rates of U87-MG cells in each group.The qRT-PCR method was used to detect the relative expression levels of miRNA-185-5p and cell division cycle 42(CDC42)mRNA in U87-MG cells of each group.Western blotting was used to detect the relative expression levels of CDC42 and N-myc proteins in U87-MG cells of each group.Results With the increase of the administration concentration of Huanggen Tablets,the survival rate of U87-MG cells gradually decreased,and its IC50 was 13 mg/mL(crude drug)at 24 hours after the intervention.The low-,medium-,and high-dose Huanggen Tablets groups had lower cell viability after 24,48,72,and 96 hours of administration,decreased relative cloning formation rate after 7 days of administration,and reduced relative migration and invasion rates after 48 hours of administration,as compared with the control group(all P<0.05).After 24 hours of administration,the relative expression levels of miRNA-185-5p in the cells of the low-,medium-,and high-dose Huanggen Tablets groups were all higher than those in the control group(all P<0.05).After 24 hours of administration,the relative expression levels of CDC42 mRNA in the low-and medium-dose Huanggen Tablets groups were lower than that in the control group(all P<0.05).After 24 hours of administration,the relative expression levels of CDC42 and N-myc proteins in the low-,medium-,and high-dose Huanggen Tablets groups were all lower than those in the control group.Conclusion Huanggen Tablets can inhibit the proliferation,migration,and invasion abilities of human glioma U87-MG cells,thus exerting an anti-glioma effect.Its mechanism may be related to promoting the expression of miRNA-185-5p,which in turn inhibits the expression of CDC42.关键词
黄根/胶质瘤/U87-MG细胞/微小RNA-185-5p/分裂周期蛋白42/抗肿瘤/机制Key words
Prismatomeris tetrandra(Roxb.)K.Schum./Glioma/U87-MG cell/MicroRNA-185-5p/Cell division cycle 42/Antitumor/Mechanism分类
临床医学引用本文复制引用
蓝晓东,李冬梅,韦桂宁,吴禄祥,梁金行,谢佳秀,蒙珍,韦娜,钟林静,刘卫延,朱涪翠,何俊慧..黄根片对胶质瘤U87-MG细胞恶性生物学行为的影响及机制研究[J].内科,2025,20(1):1-9,9.基金项目
中央引导地方科技发展资金项目(桂科ZY21195045) （桂科ZY21195045）
广西南宁市兴宁区发展改革和科学技术局重点研发计划项目(2022A10) （2022A10）
广西科技基地和人才专项(桂科AD22035055,桂科AD18216002) （桂科AD22035055,桂科AD18216002）
