中国药业2025，Vol.34Issue(10)：49-55,7.DOI:10.3969/j.issn.1006-4931.2025.10.011
指纹图谱、含量测定及化学模式识别评价三七不同部位的质量
Quality Evaluation of Different Parts of Panax notoginseng by Fingerprint,Content Determination and Chemical Pattern Recognition
摘要
Abstract
Objective To establish the high performance liquid chromatography(HPLC)fingerprint chromatogram of the rhizome,main root,branch root,and fibrous root of Panax notoginseng,and comprehensively evaluate the quality of the medicinal material.Methods HPLC was used to determine the contents of notoginsenoside R1,and ginsenosides Rg1,Re,Rb1,and Rd in the herbal samples.The chromatographic column was Waters Symmetry C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-water(gradient elution),the flow rate was 1.2 mL/min,the detection wavelength was 203 nm,the column temperature was 25 ℃,and the injection volume was 10 µL.The similarity evalution system of chromatographic fingerprint of traditional Chinese medicine was used to generate HPLC fingerprint,identify common peaks and calculate similarity values.Cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to perform chemical pattern recognition of different parts of Panax notoginseng.Results The linear ranges of concentrations of notoginsenoside R1 and ginsenosides Rg1,Re,Rb1,and Rd were in the range of 40.74-814.80 µg/mL,144.72-2 894.40 µg/mL,20.08-401.60 µg/mL,122.56-2 451.20 µg/mL,and 15.60-312.00 µg/mL(r>0.999 7).The RSDs of precis ion,stability,and repeatability tests were all lower than 2%.The total content of the five saponins in the rhizome,main root,branch root,and fibrous root was 13.01%-17.17%,8.34%-12.40%,6.97%-10.49%,and 3.53%-8.65%,respectively.A total of 14,13,13,and 13 common peaks were identified from the HPLC fingerprints of the rhizome,main root,branch root,and fibrous root,respectively,and peak 9 was a unique peak of rhizome.The similarity values of different parts of Panax notoginseng were all above 0.97.CA and PCA could only distinguish the rhizome from other parts,while OPLS-DA further classified different parts and identified six differential marker components.Conclusion The HPLC fingerprint chromatogram method combined with multivariate analysis can effectively distinguish different parts of Panax notoginseng and provide a basis for quality evaluation.关键词
三七/高效液相色谱法/指纹图谱/含量测定/化学模式识别/质量评价Key words
Panax notoginseng/high performance liquid chromatography/fingerprint chromatogram/content determination/chemical pattern recognition/quality evaluation分类
医药卫生引用本文复制引用
李秋叶,伏孟瑜,刘代涛,梁光会..指纹图谱、含量测定及化学模式识别评价三七不同部位的质量[J].中国药业,2025,34(10):49-55,7.基金项目
云南省科技计划项目[202005AF150076]. （）
