中国肿瘤生物治疗杂志2025，Vol.32Issue(4)：378-385,8.DOI:10.3872/j.issn.1007-385x.2025.04.005
CircPUM1通过miR-337-3p/NPM1轴调控子宫内膜癌Ishikawa细胞的恶性生物学行为
CircPUM1 regulates malignant biological behaviors of endometrial cancer Ishikawa cells through the miR-337-3p/NPM1 axis
摘要
Abstract
Objective:To investigate the effect of circular RNA(cireRNA)pumilio RNA binding family member 1(PUM1)on the proliferation,migration,invasion and apoptosis of endometrial cancer(EC)Ishikawa cells by regulating the miR-337-3p/nucleophosmin 1(NPM1)axis.Methods:Ishikawa cells were selected and plasmid sh-circPUM1 and its negative control(sh-NC),anti-miR-337-3p and its negative control(anti-NC)were transfected into Ishikawa cells by RNA interference.The experiment cells were divided into the control group(non-transfected cells),sh-NC group,sh-circPUM1 group,sh-circPUM1+anti-NC group and sh-circPUM1+anti-miR-337-3p group.The qPCR method was applied to detect the expressions of circPUM1,miR-337-3p,and NPM1 mRNA in Ishikawa cells in each group.CCK-8 method,EdU staining method,Transwell assay,and flow cytometry were applied respectively to detect the effects of knocking down circPUM1 on the proliferation,migration,invasion and apoptosis of Ishikawa cells.Western blot was applied to detect the changes in the expressions of PCNA,NPM1,MMP-9,SNAIL,E-cadherin,BAX and C-caspase-3 proteins in Ishikawa cells.Dual luciferase reporter gene experiment was applied to verify the targeting relationship between circPUM1 and miR-337-3p,and between miR-337-3p and NPM1.Results:Cell proliferation ability,EdU positive cell rate,migration and invasion numbers,circPUM1,NPM1 mRNA and protein,the expressions of PCNA,NPM1,MMP-9 and SNAIL protein in Ishikawa cells in the sh-circPUM1 group were significantly lower than those in the sh-NC group and Control group(all P<0.05);the apoptosis rate,the expressions of miR-337-3p,E-cadherin,BAX,and C-caspase-3 proteins were significantly higher than those in the sh-NC group and the Control group(all P<0.05).Compared with those in the sh-circPUM1 group and the sh-circPUM1+anti-NC group,the apoptosis rate,miR-337-3p,the expressions of E-cadherin,BAX,and C-caspase-3 proteins in the sh-circPUM1+anti-miR-337-3p group were significantly lower(all P<0.05);Cell proliferation ability,EdU positive cell rate,migration and invasion numbers,NPM1 mRNA and protein,the expressions of PCNA,NPM1,MMP-9,and SNAIL proteins were significantly higher(all P<0.05).CircPUM1 might target and negatively regulate miR-337-3p,and miR-337-3p might target and negatively regulate NPM1.Conclusion:Knocking down circPUM1 can inhibit the malignant biological behavior of Ishikawa cells,which might be achieved by targeting the miR-337-3p/NPM1 axis.关键词
环状RNA/pumilio RNA结合家族成员1/miR-337-3p/核磷蛋白1/子宫内膜癌/Ishikawa细胞/增殖/迁移/侵袭/凋亡Key words
circular RNA(circRNA)/pumilio RNA binding family member 1(PUM1)/miR-337-3p/nucleophosmin 1(NPM1)/endometrial cancer(EC)/Ishikawa cell/proliferation/migration/invasion/apoptosis分类
临床医学引用本文复制引用
万亚丽,吴云龙,詹磊..CircPUM1通过miR-337-3p/NPM1轴调控子宫内膜癌Ishikawa细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2025,32(4):378-385,8.基金项目
安徽医科大学2020年度科研基金项目(No.2020xkj058) （No.2020xkj058）
